Frame L T, Yeung S M, Venter J C, Cooper D M
Biochem J. 1986 Apr 15;235(2):621-4. doi: 10.1042/bj2350621.
The adenosine receptor of rat cerebral-cortical membranes was examined by radiation inactivation. In control membranes the receptor is distributed between high- and low-affinity states, that can be preferentially expressed by Mg2+ ions and guanine nucleotides respectively. Upon exposure to increasing doses of radiation, the high-affinity receptor decayed linearly as a function of radiation dose. This decay rate corresponded to a target size of 63,000 Da, when compared with the decay of the muscarinic cholinergic receptor that was also measured in these membranes.
通过辐射失活法检测大鼠大脑皮层膜中的腺苷受体。在对照膜中，该受体分布于高亲和力和低亲和力状态之间，分别可由Mg2+离子和鸟嘌呤核苷酸优先表达。随着辐射剂量增加，高亲和力受体随辐射剂量呈线性衰减。与同样在这些膜中测量的毒蕈碱型胆碱能受体的衰减相比，这种衰减速率对应于63,000 Da的靶标大小。
