含重组血小板衍生生长因子-BB的合成硫酸藻酸盐水凝胶对伤口愈合的影响。
The effect of synthetic alginate sulfate hydrogels with recombinant PDGF-BB on Wound healing.
作者信息
Babavalian H, Tebyanian H, Latifi A M, Shokrgozar M A, Bonakdar S, Shakeri F
出版信息
Bratisl Lek Listy. 2018;119(6):391-396. doi: 10.4149/BLL_2018_072.
OBJECTIVE
Platelet-derived growth factor (PDGF-BB) is an important factor in the regeneration and wound healing. One of the problems is that there is not enough efficiency in the transmission or delivery of such factors in the wound site. In this study, alginate sulfate hydrogel was synthesized with recombinant PDGF-BB growth factor for achieving a quick method in wound healing.
METHODS
In this study, Alginate sulfate hydrogel was made by photo-crosslinking method and methacrylate. Its toxicity was evaluated by MTT assay. Transforming growth factor was studied in releasing from synthesized alginate sulfate hydrogels and also, lack of toxicity was confirmed, and hydrogel was made with a recombinant human growth factor. Wounds were created with a diameter of 10 mm on the back of rats in order to check the wound healing.
RESULTS
This study showed that alginate sulfate hydrogels-PDGF-BB were faster in wound healing than non-sulfate alginate hydrogels-PDGF-BB. Therefore, the controlled delivery of growth factor system can be a powerful idea for therapeutic applications for wound healing.
CONCLUSION
Alginate sulfate hydrogel with recombinant growth factor 4 µg/cm2 (PDGF-BB) was very suitable for wound healing (Tab. 1, Fig. 3, Ref. 34).
目的
血小板衍生生长因子（PDGF - BB）是再生和伤口愈合中的一个重要因子。其中一个问题是此类因子在伤口部位的传递或递送效率不足。在本研究中，合成了硫酸藻酸盐水凝胶与重组PDGF - BB生长因子，以实现一种快速的伤口愈合方法。
方法
在本研究中，硫酸藻酸盐水凝胶通过光交联法和甲基丙烯酸酯制成。通过MTT法评估其毒性。研究了转化生长因子从合成的硫酸藻酸盐水凝胶中的释放情况，并且证实了其无毒性，还用重组人生长因子制成了水凝胶。在大鼠背部制造直径为10毫米的伤口，以检查伤口愈合情况。
结果
本研究表明，硫酸藻酸盐水凝胶 - PDGF - BB在伤口愈合方面比非硫酸藻酸盐水凝胶 - PDGF - BB更快。因此，生长因子系统的可控递送对于伤口愈合的治疗应用可能是一个有力的想法。
结论
含4 µg/cm²重组生长因子（PDGF - BB）的硫酸藻酸盐水凝胶非常适合伤口愈合（表1，图3，参考文献34）。
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