Lyu Jiao, Zhang Qi, Jin Haiying, Xu Yu, Chen Chunli, Ji Xunda, Zhang Xiang, Rao Yuqing, Zhao Peiquan
Department of Ophthalmology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, 1665 Kong Jiang Road, Shanghai, 200092, China.
Department of Ophthalmology, Shengli Oilfield Central Hospital, Dongying, Shan Dong Province, China.
Graefes Arch Clin Exp Ophthalmol. 2018 Aug;256(8):1469-1477. doi: 10.1007/s00417-018-4034-5. Epub 2018 Jun 9.
To determine the aqueous humor levels of cytokines in eyes with type 1 retinopathy of prematurity (ROP) before primary intravitreal injection of ranibizumab (IVR).
Forty-nine infants with type 1 ROP (56 eyes of 28 infants in the threshold ROP group and 42 eyes of 21 infants in the type 1 pre-threshold ROP group) received primary IVR and 49 aqueous humor samples were obtained preoperatively. Aqueous humor samples from 15 infants (15 eyes) undergoing congenital cataract surgery were used as controls. The concentrations of 27 cytokines were measured by a multiplex bead assay. Infants with persistent, recurrent, or progressive ROP after IVR were retreated.
The preoperative aqueous levels of 16 cytokines were significantly different among type 1 pre-threshold, threshold ROP, and control groups (P < 0.05). The concentrations of vascular endothelial growth factor (VEGF) (P < 0.001), interferon-γ (P < 0.001), interleukin (IL)-10 (P < 0.001), and IL-12 (P < 0.001) were the highest in the threshold ROP group, less in the type 1 pre-threshold ROP group, and the lowest in the control group. Retreatment was given to 55% of infants with ROP within a 48-week follow-up period after primary IVR. Higher VEGF (hazard ratio [HR] = 1.001, P = 0.001) and macrophage inflammatory protein-1β (HR = 1.085, P = 0.022) levels were independently correlated with ROP retreatment.
Higher aqueous levels of VEGF and inflammatory cytokines were associated with more severe type 1 ROP and ROP retreatment after primary IVR.
确定在初次玻璃体内注射雷珠单抗（IVR）前，1型早产儿视网膜病变（ROP）患儿眼内房水中细胞因子的水平。
49例1型ROP患儿（阈值ROP组28例患儿的56只眼，1型阈值前ROP组21例患儿的42只眼）接受了初次IVR，并在术前获取了49份房水样本。将15例接受先天性白内障手术的婴儿（15只眼）的房水样本用作对照。通过多重微珠分析法测量27种细胞因子的浓度。IVR后患有持续性、复发性或进行性ROP的婴儿接受再次治疗。
16种细胞因子的术前房水水平在1型阈值前、阈值ROP和对照组之间存在显著差异（P < 0.05）。血管内皮生长因子（VEGF）（P < 0.001）、干扰素-γ（P < 0.001）、白细胞介素（IL）-10（P < 0.001）和IL-12（P < 0.001）的浓度在阈值ROP组中最高，在1型阈值前ROP组中较低，在对照组中最低。在初次IVR后的48周随访期内，55%的ROP患儿接受了再次治疗。较高的VEGF（风险比[HR] = 1.001，P = 0.001）和巨噬细胞炎性蛋白-1β（HR = 1.085，P = 0.022）水平与ROP再次治疗独立相关。
较高的房水VEGF和炎性细胞因子水平与更严重的1型ROP以及初次IVR后的ROP再次治疗相关。
