Graduate School of Agricultural Science, Tohoku University, 468-1, Aramaki-Aza- Aoba, Sendai, 980-0845, Japan.
Department of Virus and Phytoplasma, Plant Pathology Research Institute, Agriculture Research Center, Giza, 12619, Egypt.
Arch Virol. 2018 Oct;163(10):2835-2840. doi: 10.1007/s00705-018-3909-4. Epub 2018 Jun 14.
Low-temperature atmospheric-pressure air plasma is a source of charged and neutral gas species. In this study, N-carrying tobacco plants were inoculated with plasma irradiated and non-irradiated tobacco mosaic virus (TMV) solution, resulting in necrotic local lesions on non-irradiated, but not on irradiated, TMV-inoculated leaves. Virus particles were disrupted by plasma irradiation in an exposure-dependent manner, but the viral coat protein subunit was not. TMV RNA was also fragmented in a time-dependent manner. These results indicate that plasma irradiation of TMV can collapse viral particles to the subunit level, degrading TMV RNA and thereby leading to a loss of infectivity.
低温常压空气等离子体是带电荷和中性气体物质的源头。在这项研究中，用等离子体辐照和未辐照的烟草花叶病毒（TMV）溶液接种含氮烟草植株，结果发现，未辐照的 TMV 接种叶片出现了坏死的局部病变，但辐照的 TMV 接种叶片没有。病毒颗粒随着暴露时间的增加而被等离子体辐照破坏，但病毒外壳蛋白亚基没有被破坏。TMV RNA 也随着时间的推移而片段化。这些结果表明，TMV 的等离子体辐照可以将病毒颗粒瓦解到亚基水平，降解 TMV RNA，从而导致其丧失感染性。
