Liu Wei, Liu Xiaoxu, Wu Changwen, Jiang Lihua
National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
Fish Physiol Biochem. 2018 Oct;44(5):1333-1347. doi: 10.1007/s10695-018-0525-x. Epub 2018 Jun 15.
The large yellow croaker (Larimichthys crocea) has low hypoxia tolerance compared with other fish species, and the mRNA levels of hypoxia-inducible factor (HIF)-1α in its brain do not change markedly under hypoxic conditions. In this study, we investigated noncoding transcription in the hypoxic response mechanism of L. crocea. We generated a catalog of long noncoding RNAs (lncRNAs) from the brain of L. crocea individuals under hypoxic stress, investigated lncRNA expression patterns, and analyzed the HIF signaling pathway by RNA sequencing. Prolyl hydroxylase domain 2 (PHD2) expression significantly increased after 6 and 12 h of hypoxia, and a lncRNA (Linc_06633.1) was found in the upstream, antisense region of PHD2. Linc_06633.1 may be an important regulator that promotes PDH2 expression under hypoxia in L. crocea, and we constructed a regulatory profile of L. crocea under hypoxic conditions. To the best of our knowledge, it is the first study that has been conducted on hypoxia signaling pathway regulation by lncRNAs in L. crocea and elucidates the role played by lncRNAs in the regulation of the hypoxia stress response in teleost fish.
与其他鱼类相比，大黄鱼（Larimichthys crocea）的耐缺氧能力较低，其大脑中缺氧诱导因子（HIF）-1α的mRNA水平在缺氧条件下无明显变化。在本研究中，我们调查了大黄鱼缺氧反应机制中的非编码转录情况。我们通过RNA测序，从缺氧应激下的大黄鱼个体大脑中生成了一个长链非编码RNA（lncRNA）目录，研究了lncRNA的表达模式，并分析了HIF信号通路。缺氧6小时和12小时后，脯氨酰羟化酶结构域2（PHD2）的表达显著增加，并且在PHD2的上游反义区域发现了一个lncRNA（Linc_06633.1）。Linc_06633.1可能是促进大黄鱼在缺氧条件下PHD2表达的重要调节因子，我们构建了大黄鱼在缺氧条件下的调控图谱。据我们所知，这是首次关于lncRNAs对大黄鱼缺氧信号通路调控的研究，并阐明了lncRNAs在硬骨鱼类缺氧应激反应调控中所起的作用。
