Wu Yang, Zhang Wei, Xu Di, Ding Li, Ma Rong, Wu Jian-Zhong, Tang Jin-Hai
Research Center of Clinical Oncology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, People's Republic of China.
Department of General Surgery, The First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, People's Republic of China.
Lasers Med Sci. 2018 Sep;33(7):1601-1608. doi: 10.1007/s10103-018-2532-7. Epub 2018 Jun 9.
In this work, a novel photothermal agent based on methionine (Met) was synthesized, which shows strong absorbance in the near infrared ray (NIR) region and is available for NIR imaging and in vivo photothermal therapy in a mouse model. Comparing to free IR-782, the obtained Met modified fluorescent dye (Met-IR-782) exhibited excellent fluorescence stability, preferable photothermal conversion efficiency under 780 nm laser irradiation and specific targeting to MCF7 (human breast adenocarcinoma cell line) cells. The fluorescence imaging ability enabled in situ monitoring of the tumor accumulation of Met-IR-782. The photothermal cytotoxicity assays in vitro and photothermal therapy treatments in vivo indicated that Met-IR-782 could efficiently target and suppress the growth of MCF7 xenograft tumors. Hence, Met-IR-782 is a potential fluorescent agent for NIR imaging-guided cancer photothermal therapy in clinical application. This work highlights the prospect of using light absorbing agents for NIR imaging-guided photothermal therapy.
在本研究中，合成了一种基于蛋氨酸（Met）的新型光热剂，其在近红外（NIR）区域具有强吸收能力，可用于小鼠模型的近红外成像和体内光热治疗。与游离的IR-782相比，所获得的Met修饰荧光染料（Met-IR-782）表现出优异的荧光稳定性，在780 nm激光照射下具有较好的光热转换效率，并且对MCF7（人乳腺腺癌细胞系）细胞具有特异性靶向作用。荧光成像能力使得能够原位监测Met-IR-782在肿瘤中的蓄积情况。体外光热细胞毒性试验和体内光热治疗表明，Met-IR-782能够有效地靶向并抑制MCF7异种移植瘤的生长。因此，Met-IR-782是临床应用中用于近红外成像引导癌症光热治疗的一种潜在荧光剂。这项工作突出了使用光吸收剂进行近红外成像引导光热治疗的前景。
