Chen W R, Adams R L, Higgins A K, Bartels K E, Nordquist R E
Oklahoma School of Science and Mathematics, Oklahoma City 73104, USA.
Cancer Lett. 1996 Jan 2;98(2):169-73.
Murine mammary tumors were treated using indocyanine green and an 808 nm diode laser, and the in vivo chromophore-enhanced photothermal effects on the tumor burden and on tumor rat survival were investigated. The power of the laser was selected in the range of 5-10 W, and irradiation duration 3-5 min. One percent aqueous indocyanine green solution in a volume of 100-200 microliters was administered in situ, either acutely or 24 h prior to the treatment. The photothermal interaction was apparent under all our treatment conditions with a well-defined spatial containment in this study and the tumor growth was slowed after treatment. The post-treatment observation showed tumor recurrence and metastasis; no long-term survival was achieved with the single application of laser in conjunction with indocyanine green. Our results pose a question on the efficacy of the photothermal interaction even though tumor cell destruction can be achieved in a large and controlled scale. However, this highly selective photothermal impact on the tumor tissue did suggest that this method be applied repeatedly to be more effective and be used as the precursor of other modalities, such as chemotherapy, radiation therapy, immunotherapy, and surgery.
使用吲哚菁绿和808纳米二极管激光对小鼠乳腺肿瘤进行治疗，并研究了体内生色团增强的光热效应对肿瘤负荷和荷瘤大鼠存活情况的影响。激光功率选择在5 - 10瓦范围内，照射持续时间为3 - 5分钟。在治疗前即刻或提前24小时原位注射100 - 200微升体积的1%吲哚菁绿水溶液。在本研究中，在所有治疗条件下光热相互作用均很明显，且具有明确的空间限制，治疗后肿瘤生长减缓。治疗后的观察显示肿瘤复发和转移；单独应用激光联合吲哚菁绿未实现长期存活。尽管可以在大规模且可控的范围内实现肿瘤细胞破坏，但我们的结果对光热相互作用的疗效提出了疑问。然而，这种对肿瘤组织的高度选择性光热作用确实表明，该方法应重复应用以提高有效性，并用作化疗、放疗、免疫治疗和手术等其他治疗方式的前期准备手段。
