Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.
Medicine Hospital Imaging Center, Wei fang traditional Chinese hospital, Shandong, 261000, China.
Environ Sci Pollut Res Int. 2018 Aug;25(24):24230-24240. doi: 10.1007/s11356-018-2503-3. Epub 2018 Jun 13.
With the widespread use of natural pyrethrins and pyrethroids to defend pest insects, people had the sustained interest in the potential risk to environment and human health. However, the research about natural pyrethrins and beta-cypermethrin induction of cytotoxicity is still seldom. This study is about the cytotoxic effects of these on human non-target cells in vitro. The cytotoxic effect of natural pyrethrins and beta-cypermethrin on QSG7701 cells were researched by using various bioassays in vitro. The results suggested that with the natural pyrethrin concentration increased, the viability of QSG7701 cells were inhibited increasingly, and the IC value as calculated was approximately 42.54 and 18.68 μg/mL after the cells were treated 24 and 48 h. The proliferative potential of QSG7701 cells treated with 40 μg/mL natural pyrethrins 6 and 12 h was decreased by 67.44 and 94.74%, dramatic enhancement ROS, collapse of mitochondrial membrane potential, DNA exhibit severity of impairment, and chromatin DNA condensation. However, beta-cypermethrin has lower toxicity than natural pyrethrins. The IC values of beta-cypermethrin were all > 80 μg/mL, and the colony formation expression was decreased by 15.26 and 19.09%, which implied that natural pyrethrins are more significantly cytotoxic and potentially genotoxic to human hepatocyte cells.
随着天然除虫菊酯和拟除虫菊酯在防治害虫方面的广泛应用，人们对其潜在的环境和人类健康风险持续关注。然而，关于天然除虫菊酯和β-氯氰菊酯诱导细胞毒性的研究仍然很少。本研究旨在探讨这两种物质对体外人非靶细胞的细胞毒性作用。通过体外多种生物测定方法研究了天然除虫菊酯和β-氯氰菊酯对 QSG7701 细胞的细胞毒性作用。结果表明，随着天然除虫菊酯浓度的增加，QSG7701 细胞的活力逐渐受到抑制，细胞分别处理 24 和 48 h 后，IC 值约为 42.54 和 18.68 μg/mL。经 40 μg/mL 天然除虫菊酯处理 6 和 12 h 后，QSG7701 细胞的增殖潜力分别降低了 67.44%和 94.74%，ROS 显著增加，线粒体膜电位崩溃，DNA 严重受损，染色质 DNA 凝聚。然而，β-氯氰菊酯的毒性比天然除虫菊酯低。β-氯氰菊酯的 IC 值均>80 μg/mL，集落形成表达降低了 15.26%和 19.09%，这表明天然除虫菊酯对人肝细胞的细胞毒性和潜在遗传毒性更为显著。
