Zhang Renyi, Wang Xue
School of Life Sciences, Guizhou Normal University, 116 Baoshan Road, Guiyang, 550001, Guizhou, China.
Institute of Fisheries, Guizhou Academy of Agriculture Science, Guiyang, 550006, Guizhou, China.
Genes Genomics. 2018 Oct;40(10):1033-1040. doi: 10.1007/s13258-018-0711-3. Epub 2018 Jun 8.
The genus Sinocyclocheilus is a representative group of cave creatures. However, genetic studies on Sinocyclocheilus are rare. The primary objective of this study was to explore the structure and feature of the complete mitochondrial genome of S. multipunctatus, and reconstruct the mitogenomic phylogeny of Sinocyclocheilus. The mitochondrial DNA of S. multipunctatus was amplified by overlapping PCR fragments. The mitogenome was assembled by the SeqMan and annotated using MitoAnnotator. The phylogenetic tree was established using the Bayesian inference and Maximum likelihood methods. The mitogenome of S. multipunctatus is a typical circular molecule of 16,586 bp with base composition A (31.25%), T (25.90%), G (16.35%), and C (26.50%), and consists of 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs) genes, two ribosomal RNAs, and a 931 bp control region. Phylogenetic analysis reveals two clades in the Sinocyclocheilus with robust support. S. multipunctatus is close to a newly discovered cavefish, S. ronganensis. We obtained and described the complete mitogenome of S. multipunctatus, and investigated its phylogenetic status, which may provide a valuable resource for future phylogenetic analyses and population genetic studies in Sinocyclocheilus.
金线鲃属是洞穴生物的一个代表性类群。然而，关于金线鲃的遗传学研究却很少。本研究的主要目的是探究多斑金线鲃完整线粒体基因组的结构和特征，并重建金线鲃属的线粒体基因组系统发育树。通过重叠PCR片段扩增多斑金线鲃的线粒体DNA。利用SeqMan组装线粒体基因组，并使用MitoAnnotator进行注释。采用贝叶斯推断和最大似然法构建系统发育树。多斑金线鲃的线粒体基因组是一个典型的环状分子，长度为16,586 bp，碱基组成为A（31.25%）、T（25.90%）、G（16.35%）和C（26.50%），由13个蛋白质编码基因（PCGs）、22个转运RNA（tRNAs）基因、两个核糖体RNA和一个931 bp的控制区组成。系统发育分析揭示了金线鲃属中有两个得到有力支持的分支。多斑金线鲃与新发现的洞穴鱼隆安金线鲃亲缘关系较近。我们获得并描述了多斑金线鲃的完整线粒体基因组，并研究了其系统发育地位，这可能为金线鲃属未来的系统发育分析和群体遗传学研究提供有价值的资源。
