Fülöp T, Fóris G, Wórum I, Leövey A
Clin Exp Immunol. 1985 Aug;61(2):425-32.
Changes in the effector functions in polymorphonuclear leucocytes (PMNL), harvested from blood of young and aged healthy subjects of both sexes, were studied. FC gamma-receptor (Fc gamma R)-mediated incorporation of IgG coated 51Cr-HRBC significantly increased in the aged male group, while the phagocytosis of pre-opsonized fungi (Saccharomyces cerevisiae and Candida albicans) was independent of both the age and sex. However, the intracellular killing capacity of neutrophils obtained from aged male subjects significantly decreased toward 51Cr-labelled c. albicans. The antibody-dependent cellular cytotoxicity (ADCC) was also impaired with ageing in both sexes. The age-dependent decrease in the effector functions of PMNL may be explained, among others, by the fact that during yeast cell incorporation the increased cAMP level does not return to the basic level in the old group. On the other hand, the cGMP level which increased in PMNL of aged subjects does not show any progressive increase as in the young subjects, but remains unchanged. The oxidative metabolism producing free radicals being necessary for the effective intracellular killing and ADCC diminished in PMNL of aged subjects of both sexes. The above findings indicate that the adaptation of cyclic nucleotide system and the oxidative burst to the cell activation becomes impaired with ageing.
研究了从年轻和老年健康男女受试者血液中采集的多形核白细胞（PMNL）效应功能的变化。在老年男性组中，Fcγ受体（FcγR）介导的IgG包被的51Cr-HRBC掺入显著增加，而预调理真菌（酿酒酵母和白色念珠菌）的吞噬作用与年龄和性别均无关。然而，老年男性受试者的中性粒细胞对51Cr标记的白色念珠菌的细胞内杀伤能力显著降低。两性的抗体依赖性细胞毒性（ADCC）也随着年龄增长而受损。PMNL效应功能随年龄的下降，除其他因素外，可能是由于在酵母细胞掺入过程中，老年组中升高的cAMP水平未恢复到基础水平。另一方面，老年受试者PMNL中升高的cGMP水平并未像年轻受试者那样呈现任何渐进性增加，而是保持不变。产生自由基的氧化代谢对于有效的细胞内杀伤和ADCC是必需的，在两性老年受试者的PMNL中均减少。上述发现表明，随着年龄增长，环核苷酸系统和氧化爆发对细胞激活的适应性受损。
