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基于功能成分与形态指标相关性分析的米炒党参炮制规范化研究

[Standardization of preparation of rice fried Codonopsis Radix based on correlation analysis of functional component and shape index].

作者信息

Chen Jiang-Peng, Dai Jun-Dong, Pei Wen-Xuan, Pan Meng, Zhang Xin, Dong Ling, Wu Yue

机构信息

Beijing University of Chinese Medicine, Beijing 102488, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2018 Jun;43(12):2543-2551. doi: 10.19540/j.cnki.cjcmm.20180322.002.

Abstract

The quality standard of Chinese herbal medicine is the key in quality control, and the establishment of rice processing technology standard of rice fried Codonopsis Radix has important significance for the overall quality control. In this study, the color brightness (L*), red-green chromaticity coordinate (a*), yellow-blue chromaticity coordinate (b*) and total color value(E*ab)were determined by colorimeter and used as the shape indexes. Ethanol extractives, polysaccharide, lobetyolin and 5-hydroxymethyl furfural contents were determined by using high performance liquid chromatography (HPLC) and ultraviolet (UV), and used as chemical indexes in the analysis by normalization distance method. The two index methods were adopted to optimize the processing technology of rice fried Codonopsis Radix by taking processing temperature, processing time, rice types and rice dosage as factors. The correlation analysis between the two index methods was carried out by SAS 9.40 and JMP 11 software. The results showed that all the four factors had significant differences, and the best processing technology was as follows: polished round-grained rice as raw material, processing temperature at 140 °C, processing time of 10min, and the ratio of Codonopsis pilosula and rice is 100:30. Chemical indexes showed significant positive correlations with shape indexes, providing basis for the quality evaluation of rice fried Codonopsis Radix in processing.

摘要

中药质量标准是质量控制的关键,建立米炒党参的炮制工艺标准对整体质量控制具有重要意义。本研究采用色差仪测定米炒党参的颜色亮度(L*)、红绿色度坐标(a*)、黄蓝色度坐标(b*)和总色值(E*ab),作为形态指标。采用高效液相色谱法(HPLC)和紫外分光光度法(UV)测定乙醇提取物、多糖、党参炔苷和5-羟甲基糠醛的含量,作为归一化距离法分析中的化学指标。以加工温度、加工时间、米的种类和米的用量为因素,采用两种指标方法优化米炒党参的炮制工艺。利用SAS 9.40和JMP 11软件对两种指标方法进行相关性分析。结果表明,4个因素均有显著差异,最佳炮制工艺为:以粳米为原料,加工温度140℃,加工时间10min,党参与米的比例为100∶30。化学指标与形态指标呈显著正相关,为米炒党参炮制过程中的质量评价提供了依据。

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