禽类MC29和MC31病毒癌蛋白的比较。
Comparison of avian MC29 and MC31 viral onc proteins.
作者信息
Sovová V, Todorov T G, Ivanov I, Dimitrov T V, Sulová A, Cerná H, Dostálová V, Hlozánek I, Mladenov Z
出版信息
Folia Biol (Praha). 1985;31(4):297-302.
Lysates of [35S]methionine-labelled quail cells transformed by MC31 virus were immunoprecipitated with anti-myc or anti-gag serum and analysed in SDS-PAGE. A protein with a molecular weight 110K was found in both immunoprecipitates. Thus, the product of the MC31 genome is a gag-myc fusion protein with the molecular weight as the product of the MC29 genome. Comparison by tryptic peptide mapping and p15 cleavage analysis showed no differences between both P110 proteins.
用抗 myc 或抗 gag 血清对经 MC31 病毒转化的[35S]甲硫氨酸标记的鹌鹑细胞裂解物进行免疫沉淀，并在 SDS-PAGE 中进行分析。在两种免疫沉淀物中均发现了一种分子量为 110K 的蛋白质。因此，MC31 基因组的产物是一种 gag-myc 融合蛋白，其分子量与 MC29 基因组的产物相同。通过胰蛋白酶肽图谱分析和 p15 裂解分析比较表明，两种 P110 蛋白之间没有差异。
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