禽MC29和OK10病毒的myc特异性蛋白中p15裂解位点的证据。
Evidence for p15 cleavage site in myc-specific proteins of avian MC29 and OK10 viruses.
作者信息
Sovová V, Trávnicek M, Hlozánek I, Cerná H, Alitalo K, Vaheri A
出版信息
J Cell Biochem. 1985;28(4):265-72. doi: 10.1002/jcb.240280404.
Myc-related proteins were precipitated from MC29 virus-transformed cells (PR-2) and from OK10 virus-transformed cells (9C) by anti-gag and anti-myc sera. Immunoprecipitates were cleaved with the avian retroviral protease p15 and the cleavage products analyzed in SDS-PAGE. Cleavage fragments of p110gag-myc (product of MC29 virus) and p58myc (product of OK10 virus) showed the presence of a p15 cleavage site within the myc-specific region. The site is missing in deletion mutants of MC29 virus.
通过抗gag血清和抗myc血清，从MC29病毒转化细胞（PR-2）和OK10病毒转化细胞（9C）中沉淀出与Myc相关的蛋白质。用禽逆转录病毒蛋白酶p15切割免疫沉淀物，并在SDS-PAGE中分析切割产物。p110gag-myc（MC29病毒产物）和p58myc（OK10病毒产物）的切割片段显示在myc特异性区域内存在一个p15切割位点。该位点在MC29病毒的缺失突变体中不存在。
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