用蛋白酶p15体外切割分析禽成髓细胞瘤病毒MC29株部分转化缺陷型突变体的gag-myc蛋白：融合蛋白的myc结构域中存在特异性切割位点p15的证据
Analysis of gag-myc proteins of partially transformation-defective mutants of avian myelocytomatosis virus strain MC29 by in vitro cleavage with protease p15: indication for the presence of specific cleavage site p15 in the myc domain of fusion protein.
作者信息
Sovová V, Trávnícek M, Hlozánek I, Cerná H, Sulová A
出版信息
Folia Biol (Praha). 1984;30(3):145-51.
Gag-related proteins were immunoprecipitated from [35S]methionine-labelled cells of the PR-2 line which contained p110 protein as well as from quail cells transformed by deletion mutants 10A, 10C, and 10H of MC29 virus. Immunoprecipitates were incubated with oncoviral protease p15 and cleavage products were analyzed in SDS-PAGE. The major 56K fragment (F56) of p110 was further analyzed by tryptic peptide mapping. The results showed that except for the myc domain of p110, a portion of p27 is also present in F56. Cleavage of 100K, 95K, and 90K proteins coded by three MC29 deletion mutants resulted in major fragments 66K, 60K, and 56K, respectively. The existence of further cleavage fragments and presence of the p15 specific cleavage site in the myc domain of MC29 specific proteins is discussed.
从含有p110蛋白的PR - 2系[35S]甲硫氨酸标记细胞以及由MC29病毒的缺失突变体10A、10C和10H转化的鹌鹑细胞中免疫沉淀出与Gag相关的蛋白。将免疫沉淀物与肿瘤病毒蛋白酶p15一起孵育，并在SDS - PAGE中分析裂解产物。通过胰蛋白酶肽图谱进一步分析p110的主要56K片段（F56）。结果表明，除了p110的myc结构域外，F56中还存在一部分p27。由三个MC29缺失突变体编码的100K、95K和90K蛋白的裂解分别产生了主要片段66K、60K和56K。文中讨论了MC29特异性蛋白的myc结构域中进一步裂解片段的存在以及p15特异性裂解位点的情况。
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