Malysh Julia M, Ignatieva Anastasia N, Artokhin Konstantin S, Frolov Andrei N, Tokarev Yuri S
All-Russian Institute of Plant Protection, Podbelskogo 3, Pushkin, St. Petersburg, 196608, Russia.
Research Advisory Center "Agroliga" Rostov Branch, Chekhova 71, Rostov-on-Don, 344010, Russia.
Parasitol Res. 2018 Sep;117(9):3039-3044. doi: 10.1007/s00436-018-5987-3. Epub 2018 Jun 27.
Three species of Microsporidia were identified from a population of the beet webworm Loxostege sticticalis at prevalence rates of 35, 4, and 3%. The most prevalent parasite (Tubulinosema sp.) was similar to Tubulinosema acridophagus (99.8% ssrDNA sequence similarity) and was also isolated from the parasitoid Lydella thompsoni (Diptera, Tachinidae) that emerged from the beet webworms. In laboratory assays, spores of this Tubulinosema sp. showed an infection rate of up to 80% for both L. sticticalis and Galleria mellonella larvae. The spores were viable after 12 months of storage in dried infected cadavers. The second most prevalent parasite was closely related to Nosema furnacalis and Nosema granulosis (98.7% similarity). Fresh spores showed a 50% infection rate under laboratory conditions. The third most abundant parasite was identified as the honeybee pathogen Nosema ceranae (100% ssrDNA and 95-100% IGS similarity). In the laboratory, fresh spores of N. ceranae isolated from beet webworm and honey bee were infective to L. sticticalis larvae at the rates of 5 and 2%, respectively.
从草地螟种群中鉴定出三种微孢子虫，其感染率分别为35%、4%和3%。最常见的寄生虫（Tubulinosema属）与嗜蝗小管微孢子虫相似（单链核糖体DNA序列相似度为99.8%），并且也从寄生于草地螟的汤普森氏厉寄蝇（双翅目，寄蝇科）中分离得到。在实验室试验中，这种Tubulinosema属的孢子对草地螟幼虫和大蜡螟幼虫的感染率高达80%。在干燥的受感染尸体中储存12个月后，孢子仍具活力。第二常见的寄生虫与玉米螟微孢子虫和颗粒微孢子虫密切相关（相似度为98.7%）。新鲜孢子在实验室条件下的感染率为50%。第三丰富的寄生虫被鉴定为蜜蜂病原体东方蜜蜂微孢子虫（单链核糖体DNA相似度为100%，间隔区序列相似度为95 - 100%）。在实验室中，从草地螟和蜜蜂中分离出的新鲜东方蜜蜂微孢子虫孢子对草地螟幼虫的感染率分别为5%和2%。
