Increase in L929 cell interferon production in serum-free media.

Conditions are described for the production of mouse interferon by L929 cells cultured as monolayer in roller tubes. Kinetics of interferon production are described. Release of interferon into the culture medium began at 9 h after induction. One hour earlier the nutrient medium (MEM with 10% calf serum) was replaced with serum-free medium (MEM or PBS). Longer time of intensive nutrition of cells influenced the IFN yield. Interferon titers obtained with this method were higher than those obtained with the routine method of interferon production in which the nutrient media were replaced into serum-free media immediately after induction.