Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
Laboratory of Biologically Active Compounds, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
J Biophotonics. 2018 Nov;11(11):e201800079. doi: 10.1002/jbio.201800079. Epub 2018 Jul 18.
Pseudomonas aeruginosa is a common causative bacterium of acute and chronic infections that have been responsible for high mortality over the past decade. P. aeruginosa produces many virulence factors such as toxins, enzymes and dyes that are strongly dependent on quorum sensing (QS) signaling systems. P. aeruginosa has three major QS systems (las, rhl and Pseudomonas quinolone signal) that regulate the expression of genes encoding virulence factors as well as biofilm production and maturation. Antimicrobial blue light (aBL) is considered a therapeutic option for bacterial infections and has other benefits, such as reducing bacterial virulence. Therefore, this study investigated the efficacy of aBL to reduce P. aeruginosa pathogenicity. aBL treatment resulted in the reduced activity of certain QS signaling molecules in P. aeruginosa and inhibited biofilm formation. in vivo tests using a Caenorhabditis elegans infection model indicated that sublethal aBL decreased the pathogenicity of P. aeruginosa. aBL may be a new virulence-targeting therapeutic approach.
铜绿假单胞菌是一种常见的致病菌，可引起急性和慢性感染，在过去十年中导致了高死亡率。铜绿假单胞菌产生许多毒力因子，如毒素、酶和染料，这些毒力因子强烈依赖群体感应（QS）信号系统。铜绿假单胞菌有三个主要的 QS 系统（las、rhl 和铜绿假单胞喹诺酮信号），它们调节编码毒力因子以及生物膜形成和成熟的基因的表达。抗菌蓝光（aBL）被认为是治疗细菌感染的一种选择，并且具有其他益处，例如降低细菌的毒力。因此，本研究调查了 aBL 降低铜绿假单胞菌致病性的功效。aBL 处理导致铜绿假单胞菌中某些 QS 信号分子的活性降低，并抑制生物膜形成。使用秀丽隐杆线虫感染模型的体内试验表明，亚致死剂量的 aBL 降低了铜绿假单胞菌的致病性。aBL 可能是一种新的针对毒力的治疗方法。
