Yılmaz Esra Saygılı, Sapmaz Tansel, Kazgan Halil, Yildiz Şule Menziletoglu, Kocamaz Derya, Akpolat Nusret, Sapmaz Ekrem
Department of Gynecology and Obstetrics, Adana Numune Training and Research Hospital, Turkey.
Department of Histology and Embryology, Institute of Medical Sciences, Imperial School of Medicine, Istanbul, Turkey.
Adv Clin Exp Med. 2018 Jul;27(7):907-911. doi: 10.17219/acem/70810.
There is no study of whether the dysplastic changes in the ovarian surface epithelium of X-ray-exposed rats during hysterosalpingography (HSG) decrease or not with the use of Lipiodol and melatonin given both intraperitoneally (i.p.) and into the suspensorium ovarii.
We investigated the restorative effects of melatonin and Lipiodol administration during the HSG procedure on the dysplastic changes in the ovarian surface epithelium of X-ray-exposed rats.
A total of 50 Wistar rats with regular estrous cycles were randomly divided into 5 groups. Group 1 was the control group. In other groups, X-ray was applied (group 2), 0.1 mL Lipiodol was applied to each uterine horn (group 3), 20 mg/kg intraperitoneal melatonin application was followed by 0.1 mL Lipiodol administration to each uterine horn after 15 min (group 4), and 20 mg/kg melatonin was administered to the ligamentum suspensorium ovarii, followed by 0.1 mL Lipiodol application to each uterine horn after 15 min (group 5). The rats in groups 2-5 were exposed to whole body radiation 3 times. After 3 h, the abdomens of all rats were reopened and left oophorectomy was performed.
The presence of nucleoli and mitosis values were found similar among the groups. All other parameters were significantly higher in group 2 compared to other groups, except for the presence of nucleoli and mitosis values (p < 0.05). The presence of hyperchromasia and the total score were found to be the highest in group 2, followed by group 3, when compared to other groups (p < 0.05). It was detected that the detrimental effects of X-ray exposure diminished with Lipiodol use, and were further reduced by the use of melatonin in combination.
We suggest that the use of melatonin and Lipiodol during HSG may prevent the carcinogenic changes exerted by radiation on the ovarian surface epithelium.
关于子宫输卵管造影术（HSG）期间接受X射线照射的大鼠卵巢表面上皮发育异常变化是否会因腹腔内（i.p.）及卵巢悬韧带内给予碘油和褪黑素而减少，尚无相关研究。
我们研究了HSG过程中给予褪黑素和碘油对接受X射线照射的大鼠卵巢表面上皮发育异常变化的修复作用。
将50只具有规律发情周期的Wistar大鼠随机分为5组。第1组为对照组。在其他组中，第2组接受X射线照射，第3组在每个子宫角注入0.1 mL碘油，第4组腹腔注射20 mg/kg褪黑素，15分钟后在每个子宫角注入0.1 mL碘油，第5组在卵巢悬韧带内注射20 mg/kg褪黑素，15分钟后在每个子宫角注入0.1 mL碘油。第2 - 5组大鼠接受3次全身辐射。3小时后，再次打开所有大鼠的腹腔并进行左侧卵巢切除术。
各组之间核仁的存在情况和有丝分裂值相似。除核仁的存在情况和有丝分裂值外，第2组的所有其他参数均显著高于其他组（p < 0.05）。与其他组相比，第2组的核深染情况和总分最高，其次是第3组（p < 0.05）。研究发现，使用碘油可减轻X射线照射的有害影响，联合使用褪黑素可进一步降低这种影响。
我们认为，HSG期间使用褪黑素和碘油可能预防辐射对卵巢表面上皮产生的致癌性变化。
