Department of Medical Psychology, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
Department of Neurology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
Balkan Med J. 2018 Nov 15;35(6):412-416. doi: 10.4274/balkanmedj.2017.1406. Epub 2018 Jun 28.
Insomnia often coexists with depression, and there is compelling evidence for a genetic component in the etiologies of both disorders.
To investigate the relationship between exonic variant (rs4290270) in the tryptophan hydroxylase-2 gene and primary insomnia and symptoms of depression in Han Chinese.
Case-control study.
This study included 152 patients with primary insomnia and 164 age- and gender-matched normal controls. All patients were investigated by polysomnography for 2 consecutive nights. The depressive symptoms were measured by using a 20-item Zung Self-rating Depression Scale. Sleep quality was assessed with the Pittsburgh Sleep Quality index. The genotypes of the TPH-2 gene polymorphism rs4290270 were determined by the polymerase chain reaction-restriction fragment length polymorphism method.
The genotype distributions of the tryptophan hydroxylase-2 gene polymorphism rs4290270 were in Hardy-Weinberg equilibrium in both patients and controls (p>0.05). The allele and genotype distributions of this variant were comparable between patients and controls in all subjects and between genders (all p>0.05). The impact of rs4290270 on self-rating depression scale score changes was statistically significant (p=0.002), with carriers of the A/A genotype having the highest self-rating depression scale score (mean ± standard deviation: 52.73±12.88), followed by the A/T genotype (50.94±11.29, p=0.35) and the T/T genotype (43.48±7.78, p<0.01), and this impact was more obvious in women (p<0.001).
The tryptophan hydroxylase-2 gene polymorphism rs4290270 may not be a susceptibility locus for primary insomnia in Han Chinese, but it may be a marker of depressive symptoms.
失眠常与抑郁共存，这两种疾病的病因都有强有力的遗传因素证据。
调查色氨酸羟化酶-2 基因外显子变异（rs4290270）与汉族人群原发性失眠及抑郁症状的关系。
病例对照研究。
本研究纳入了 152 例原发性失眠患者和 164 名年龄和性别匹配的正常对照。所有患者均连续 2 晚接受多导睡眠图检查。使用zung 自评抑郁量表评估抑郁症状。使用匹兹堡睡眠质量指数评估睡眠质量。采用聚合酶链反应-限制性片段长度多态性方法检测 TPH-2 基因多态性 rs4290270 的基因型。
患者和对照组的色氨酸羟化酶-2 基因多态性 rs4290270 基因型分布均符合哈迪-温伯格平衡（p>0.05）。在所有受试者和性别之间，该变体的等位基因和基因型分布在患者和对照组之间无差异（均 p>0.05）。rs4290270 对自评抑郁量表评分变化的影响具有统计学意义（p=0.002），A/A 基因型携带者的自评抑郁量表评分最高（平均值±标准差：52.73±12.88），其次是 A/T 基因型（50.94±11.29，p=0.35）和 T/T 基因型（43.48±7.78，p<0.01），这种影响在女性中更为明显（p<0.001）。
色氨酸羟化酶-2 基因多态性 rs4290270 可能不是汉族人群原发性失眠的易感位点，但可能是抑郁症状的标志物。
