Sleep Medicine Center of Gansu Provincial Hospital, Lanzhou, China.
School of Chinese Traditional Medicine Integrated with Western Medicine, Gansu University of Traditional Chinese Medicine, Lanzhou, China.
Intern Med J. 2021 May;51(5):732-738. doi: 10.1111/imj.14813.
Primary insomnia is a worldwide problem and it has a considerable negative impact on one's physical and mental health. Studies have shown that non-synonymous Single-nucleotide polymorphisms in 5-hydroxytryptamine (serotonin or 5-HT) are related to primary insomnia. Previous studies have shown that 5-HT polymorphism (rs140700) is related to depression, and insomnia is often accompanied by depression and anxiety. The relationship between this site and primary insomnia is unknown. We speculated that this site may be related to primary insomnia, so we investigated the relationship between rs140700 and primary insomnia.
To explore the relationship between the 5-HT gene polymorphism rs140700 and primary insomnia.
In this study, we included 57 patients with primary insomnia and 54 age- and gender-matched normal controls. The subjects who belonged to the Chinese population were subjected to polysomnography for three consecutive nights. Their sleep quality was assessed, and the genotypes of the 5-hydroxytryptamine (5-HT) gene polymorphism rs140700 were determined by the flight mass spectrometry.
The genotype distributions of the 5-HT gene polymorphism rs140700 were in Hardy-Weinberg equilibrium in both patients and controls (P > 0.05). The allele and genotype distributions of this variant were comparable between the patients and controls in all subjects and between genders (all P > 0.05). The influence of rs140700 on percentage of stage 1 (P = 0.015) change and arousal index (P = 0.028) of primary insomnia was statistically significant. The logistic multi-factor regression analysis results revealed that 5-HT gene polymorphism rs140700 was not a risk factor for primary insomnia in the Chinese population (P = 0.589).
The 5-HT gene polymorphism rs140700 may not be a susceptibility locus for primary insomnia in the Chinese population.
原发性失眠是一个全球性的问题，它对人们的身心健康有相当大的负面影响。研究表明，5-羟色胺（血清素或 5-HT）的非 synonymous单核苷酸多态性与原发性失眠有关。先前的研究表明，5-HT 多态性（rs140700）与抑郁症有关，而失眠常伴有抑郁和焦虑。该位点与原发性失眠的关系尚不清楚。我们推测该位点可能与原发性失眠有关，因此我们研究了 rs140700 与原发性失眠的关系。
探讨 5-羟色胺基因多态性 rs140700 与原发性失眠的关系。
本研究纳入了 57 例原发性失眠患者和 54 名年龄和性别匹配的正常对照者。属于中国人群的研究对象进行了连续三个晚上的多导睡眠图检查，评估他们的睡眠质量，并通过飞行质谱法确定 5-羟色胺（5-HT）基因多态性 rs140700 的基因型。
患者和对照组的 5-HT 基因多态性 rs140700 基因型分布均符合 Hardy-Weinberg 平衡（均 P>0.05）。该变体的等位基因和基因型在所有受试者和性别之间在患者和对照组中均无差异（均 P>0.05）。rs140700 对原发性失眠患者 1 期（P=0.015）变化和觉醒指数（P=0.028）的影响具有统计学意义。多因素 logistic 回归分析结果表明，5-HT 基因多态性 rs140700 不是中国人群原发性失眠的危险因素（P=0.589）。
5-HT 基因多态性 rs140700 可能不是中国人群原发性失眠的易感位点。
