分离的哺乳动物耳蜗毛细胞的可逆性收缩

Reversible contraction of isolated mammalian cochlear hair cells.

作者信息

Zenner H P, Zimmermann U, Schmitt U

出版信息

Hear Res. 1985 May;18(2):127-33. doi: 10.1016/0378-5955(85)90004-8.

Abstract

Outer hair cells were isolated from the guinea pig cochlea using a micromechanical non-enzymatic procedure. Depolarization of outer hair cells in the presence of 25-125 mM K+ was accompanied by a longitudinal contraction of the isolated cells. A decrease of [K+] to 5.4 mM interrupted contraction and induced a relaxation. Individual hair cells were able to undergo as many as 5 cycles of contraction and relaxation. External Ca2+ was required for relaxation of the contracted hair cells. The contractile event led to the production of a visible cytoplasmic network between the supranuclear area and the cuticular plate.

摘要

采用微机械非酶法从豚鼠耳蜗中分离出外毛细胞。在25 - 125 mM K⁺存在的情况下，外毛细胞去极化伴随着分离细胞的纵向收缩。将[K⁺]降低至5.4 mM会中断收缩并诱导松弛。单个毛细胞能够经历多达5个收缩和松弛周期。收缩的毛细胞松弛需要细胞外Ca²⁺。收缩事件导致在核上区域和表皮板之间产生可见的细胞质网络。