Yen Ming-Tsung, Chang Yuan-Hua, Huang Shih-Jeng, Cheng Ming-Ching, Mau Jeng-Leun
Department of Applied Life Science and Health, Chia Nan University of Pharmacy and Science, Jenteh, Tainan, Taiwan, Republic of China.
Department of Food Science and Biotechnology, National Chung Hsing University (NCHU), Taichung, Taiwan, R.O.C.
Int J Med Mushrooms. 2018;20(4):381-392. doi: 10.1615/IntJMedMushrooms.2018025953.
Ergothioneine is an effective antioxidant and is abundant in species of genus Pleurotus. This research focused on developing an ergothioneine extract from P. eryngii and P. citrinopileatus under optimal extraction conditions. The hot-water and 70% ethanol extractions yielded more ergothioneine than microwave, ultrasonic, and autoclaving extractions. Hot-water extraction with optimal conditions-125 rpm at 75°C for 5 minutes-produced P. eryngii and P. citrinopileatus extracts with 0.86 and 3.73 mg ergothioneine/g dry weight, respectively. In addition to 50% of added maltodextrin, spray-dried products from mushrooms contained 23.07-16.58% carbohydrates and 18.32-21.40% protein. The microstructure of spray-dried products showed shrunken spheres with mean particle diameters of 15.82-19.94 μm. After in vitro simulated gastric and intestinal digestion, the spray-dried P. eryngii and P. citrinopileatus products contained 88% and 91% residual ergothioneine, respectively. Overall, the spray-dried products could be used as antioxidative supplements.
麦角硫因是一种有效的抗氧化剂，在平菇属物种中含量丰富。本研究着重于在最佳提取条件下从刺芹侧耳和金顶侧耳中开发麦角硫因提取物。热水提取和70%乙醇提取比微波提取、超声提取和高压灭菌提取得到的麦角硫因更多。在75°C、125转/分钟的最佳条件下进行5分钟的热水提取，得到的刺芹侧耳提取物和金顶侧耳提取物中麦角硫因含量分别为0.86毫克/克干重和3.73毫克/克干重。除了添加50%的麦芽糊精外，蘑菇喷雾干燥产品含有23.07 - 16.58%的碳水化合物和18.32 - 21.40%的蛋白质。喷雾干燥产品的微观结构显示为收缩的球体，平均粒径为15.82 - 19.94微米。经过体外模拟胃肠消化后，喷雾干燥的刺芹侧耳产品和金顶侧耳产品中麦角硫因的残留量分别为88%和91%。总体而言，喷雾干燥产品可用作抗氧化补充剂。
