Institute of Chemical Biology, National Hellenic Research Foundation 48, Vas. Constantinou Ave., 11635 Athens, Greece.
Department of Food Science and Technology, University of West Attica, Ag. Spyridonos, 12243 Egaleo, Greece.
Molecules. 2021 Mar 24;26(7):1832. doi: 10.3390/molecules26071832.
In recent years, mushrooms have drawn the attention of agro-industries and food-industries as they were considered to be valuable natural sources of health promoting compounds such as β-glucans, ergothioneine, and lovastatin. The detection and quantification of such compounds by implementing reliable analytical approaches is of the utmost importance in order to adjust mushrooms' cultivation conditions and maximize the production in different species. Toward this direction, the current study focuses on the comparison of ultraviolet-visible (UV-Vis) spectrometry and liquid chromatography-mass spectrometry (LC-MS) methods (a) by evaluating the content of ergothioneine and lovastatin in mushrooms and (b) by highlighting any possible substrate-based interferences that hinder the accurate determination of these two compounds in order to propose the technique-of-choice for a standardized bioactive compounds monitoring. For this purpose, mushrooms produced by three species (i.e., , , and ) on various cultivation substrates, namely wheat straw (WS), winery (grape marc (GM)), and olive oil (OL) by-products, were examined. Among the two applied techniques, the developed and validated LC-MS methods, exhibiting relatively short analysis time and higher resolution, emerge as the methods-of-choice for detecting ergothioneine and lovastatin in mushrooms. On the contrary, UV-Vis methods were hindered due to co-absorbance of different constituents, resulting in invalid results. Among the studied mushrooms, contained the highest amount of ergothioneine (822.1 ± 20.6 mg kg dry sample), whereas contained the highest amounts of lovastatin (1.39 ± 0.014 mg kg dry sample). Regarding the effect of different cultivation substrates, mushrooms produced on OL and WS contained the highest amount of ergothioneine, while mushrooms deriving from GM-based substrates contained the highest amount of lovastatin.
近年来，蘑菇因其被认为是具有β-葡聚糖、麦角硫因和洛伐他汀等有益健康化合物的宝贵天然来源而引起了农业和食品行业的关注。为了调整蘑菇的栽培条件并在不同物种中实现产量最大化，通过实施可靠的分析方法来检测和定量这些化合物至关重要。为此，本研究侧重于比较紫外可见（UV-Vis）光谱法和液相色谱-质谱（LC-MS）法（a）通过评估蘑菇中麦角硫因和洛伐他汀的含量，（b）通过突出任何可能阻碍这两种化合物准确测定的基于基质的干扰，从而提出用于标准化生物活性化合物监测的首选技术。为此，研究了在不同栽培基质（即麦草（WS）、酿酒葡萄渣（GM）和橄榄油（OL）副产物）上由三个物种（即、和）生产的蘑菇。在所应用的两种技术中，开发和验证的 LC-MS 方法，由于具有相对较短的分析时间和更高的分辨率，成为检测蘑菇中麦角硫因和洛伐他汀的首选方法。相比之下，由于不同成分的共吸收，UV-Vis 方法受到阻碍，导致结果无效。在所研究的蘑菇中，含有最高量的麦角硫因（822.1 ± 20.6 mg kg 干样品），而含有最高量的洛伐他汀（1.39 ± 0.014 mg kg 干样品）。关于不同栽培基质的影响，在 OL 和 WS 上生产的蘑菇含有最高量的麦角硫因，而源自 GM 基质的蘑菇含有最高量的洛伐他汀。
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