人乳头瘤病毒感染与胎盘、子宫颈和口腔黏膜中的细菌微生物群。
HPV infection and bacterial microbiota in the placenta, uterine cervix and oral mucosa.
机构信息
Department of Oral Pathology and Oral Radiology, Institute of Dentistry, Faculty of Medicine, University of Turku, Turku, Finland.
Department of Paediatrics, University of Turku & Turku University Hospital, Turku, Finland.
出版信息
Sci Rep. 2018 Jun 28;8(1):9787. doi: 10.1038/s41598-018-27980-3.
We investigated the association between HPV infection and bacterial microbiota composition in the placenta, uterine cervix and mouth in thirty-nine women. HPV DNA genotyping of 24 types was conducted using Multimetrix. Microbiota composition was characterized by 16S rRNA gene sequencing. HPV DNA was detected in 33% of placenta, 23% cervical and 33% oral samples. HPV16 was the most frequent type in all regions. HPV infection was associated with higher microbiota richness (p = 0.032) in the mouth but did not influence microbial diversity or richness in other samples. HPV infection was associated with higher abundance of Lactobacillaceae (p = 0.0036) and Ureaplasma (LDA score > 4.0, p < 0.05) in the placenta, Haemophilus (p = 0.00058) and Peptostreptococcus (p = 0.0069) genus in the cervix and Selenomonas spp. (p = 0.0032) in the mouth compared to HPV negative samples. These data suggest altered bacterial microbiota composition in HPV positive placenta, cervix and mouth. Whether the changes in bacterial microbiota predispose or result from HPV remains to be determined in future studies.
我们调查了 39 名女性的胎盘、子宫颈和口腔中 HPV 感染与细菌微生物群落组成之间的关系。使用 Multimetrix 进行了 24 种 HPV DNA 基因分型。通过 16S rRNA 基因测序来描述微生物群落组成。在 33%的胎盘、23%的宫颈和 33%的口腔样本中检测到 HPV DNA。所有部位最常见的 HPV 类型均为 HPV16。HPV 感染与口腔中更高的微生物丰富度相关（p=0.032），但不会影响其他样本中的微生物多样性或丰富度。HPV 感染与胎盘中乳杆菌科（p=0.0036）和脲原体（LDA 评分>4.0，p<0.05）、宫颈中嗜血杆菌（p=0.00058）和消化链球菌属（p=0.0069）以及口腔中唾液单胞菌属（p=0.0032）的丰度增加有关，与 HPV 阴性样本相比。这些数据表明 HPV 阳性的胎盘、子宫颈和口腔中的细菌微生物群落组成发生了改变。未来的研究仍需确定细菌微生物群的变化是导致 HPV 感染的原因还是结果。
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