Dar Hamid Y, Shukla Prashant, Mishra Pradyumna K, Anupam Rajaneesh, Mondal Rajesh K, Tomar Geetanjali B, Sharma Versha, Srivastava Rupesh K
Department of Zoology, School of Biological Sciences, Dr. Harisingh Gour Central University, Sagar, MP 470003, India.
Department of Physics, School of Mathematical and Physical Sciences, Dr. Harisingh Gour Central University, Sagar, MP 470003, India.
Bone Rep. 2018 Feb 5;8:46-56. doi: 10.1016/j.bonr.2018.02.001. eCollection 2018 Jun.
Osteoporosis is one of the most important but often neglected bone disease associated with aging and postmenopausal condition leading to bone loss and fragility. Probiotics have been associated with various immunomodulatory properties and have the potential to ameliorate several inflammatory conditions including osteoporosis. (LA) was selected as probiotic of choice in our present study due its common availability and established immunomodulatory properties. In the present study, we report for the first time that administration of LA in ovariectomized (ovx) mice enhances both trabecular and cortical bone microarchitecture along with increasing the mineral density and heterogeneity of bones. This effect of LA administration is due to its immunomodulatory effect on host immune system. LA thus skews the Treg-Th17 cell balance by inhibiting osteoclastogenic Th17 cells and promoting anti-osteoclastogenic Treg cells in ovx mice. LA administration also suppressed expression of osteoclastogenic factors (IL-6, IL-17, TNF-α and RANKL) and increased expression of anti-osteoclastogenic factors (IL-10, IFN-γ). Taken together the present study for the first time clearly demonstrates the therapeutic potential of LA as an osteo-protective agent in enhancing bone health (via tweaking Treg-Th17 cell balance) in postmenopausal osteoporosis.
骨质疏松症是与衰老和绝经后状况相关的最重要但常被忽视的骨病之一，会导致骨质流失和骨脆性增加。益生菌具有多种免疫调节特性，有可能改善包括骨质疏松症在内的多种炎症性疾病。在我们目前的研究中，由于嗜酸乳杆菌（LA）普遍可得且具有既定的免疫调节特性，因此被选为首选益生菌。在本研究中，我们首次报告，给去卵巢（ovx）小鼠施用LA可增强小梁骨和皮质骨的微结构，同时增加骨矿物质密度和异质性。LA施用的这种效果归因于其对宿主免疫系统的免疫调节作用。因此，LA通过抑制ovx小鼠中破骨细胞生成的Th17细胞并促进抗破骨细胞生成的调节性T细胞（Treg），从而改变Treg-Th17细胞平衡。LA施用还抑制破骨细胞生成因子（IL-6、IL-17、TNF-α和RANKL）的表达，并增加抗破骨细胞生成因子（IL-10、IFN-γ）的表达。综上所述，本研究首次明确证明了LA作为一种骨保护剂在改善绝经后骨质疏松症骨健康（通过调节Treg-Th17细胞平衡）方面的治疗潜力。
