Shirasaki Yoshitaka, Ohara Osamu
PRESTO, Japan Science and Technology Agency, Tokyo, Japan.
Department of Biological Science, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Methods Mol Biol. 2018;1808:1-7. doi: 10.1007/978-1-4939-8567-8_1.
In addition to direct physical interactions between/among cells, the secretion of humoral factors from living cells is a critical process for cell-cell communications. A well-known extracellular signaling event is mediated by immune cell cytokines/chemokines. Because cell-cell communication is crucial in immune cell sociology, protein secretion assays first attracted a broad range of immunology interests. Now that we have entered an era of systems biology, cell-cell interactions mediated by secreted molecules should be revisited to understand the dynamics and homeostasis of the cell society as a whole. Of more importance, recent advances in detection and microfluidics technologies enable us to monitor protein secretion in real time rather than as a snapshot from the past, which gives us an opportunity to more deeply understand the logic of mammalian cell sociology. This chapter reviews the recent progress in and future direction of protein secretion assays, particularly from a mammalian cell sociology viewpoint.
除了细胞之间的直接物理相互作用外，活细胞分泌体液因子是细胞间通讯的关键过程。一个著名的细胞外信号事件是由免疫细胞细胞因子/趋化因子介导的。由于细胞间通讯在免疫细胞社会学中至关重要，蛋白质分泌测定首先引起了广泛的免疫学关注。既然我们已经进入了系统生物学时代，就应该重新审视由分泌分子介导的细胞间相互作用，以了解整个细胞社会的动态和稳态。更重要的是，检测和微流控技术的最新进展使我们能够实时监测蛋白质分泌，而不是像过去那样只获取一个快照，这让我们有机会更深入地理解哺乳动物细胞社会学的逻辑。本章综述了蛋白质分泌测定的最新进展和未来方向，特别是从哺乳动物细胞社会学的角度。
