Department of Biomedical Engineering, Yale University, New Haven, Connecticut 06520, USA; email:
Yale Cancer Center, Yale Stem Cell Center, Human and Translational Immunology Program, Yale School of Medicine, New Haven, Connecticut 06520, USA.
Annu Rev Anal Chem (Palo Alto Calif). 2019 Jun 12;12(1):431-449. doi: 10.1146/annurev-anchem-061318-115055. Epub 2019 Apr 12.
Secreted proteins play important roles in mediating various biological processes such as cell-cell communication, differentiation, migration, and homeostasis at the population or tissue level. Here, we review bioanalytical technologies and devices for detecting protein secretions from single cells. We begin by discussing conventional approaches followed by detailing the latest advances in microengineered systems for detecting single-cell protein secretions with an emphasis on multiplex measurement. These platforms include droplet microfluidics, micro-/nanowell-based assays, and microchamber-based assays, among which the advantages and limitations are compared. Microscale systems also enable the tracking of protein secretion dynamics in single cells, further empowering the study of the cell-cell communication network. Looking forward, we discuss the remaining challenges and future opportunities that will transform basic research of cellular secretion functions at the systems level and the clinical applications for immune monitoring and cancer treatment.
分泌蛋白在调节细胞-细胞通讯、分化、迁移和群体或组织水平的动态平衡等各种生物学过程中发挥着重要作用。在这里，我们综述了用于检测单细胞分泌蛋白的生物分析技术和设备。我们首先讨论了传统方法，然后详细介绍了最新的微工程系统在检测单细胞蛋白分泌方面的进展，重点是对多指标的测量。这些平台包括液滴微流控、微/纳孔基检测和微腔基检测，其中比较了各自的优缺点。微尺度系统还能够跟踪单细胞中蛋白分泌的动力学，进一步增强对细胞-细胞通讯网络的研究。展望未来，我们讨论了仍需面对的挑战和未来的机遇，这些将推动系统水平的细胞分泌功能的基础研究，并为免疫监测和癌症治疗的临床应用提供新的可能。
