Department of Burns and Plastic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, P.R. China.
Department of Thoracic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, P.R. China.
Mol Med Rep. 2018 Aug;18(2):2017-2026. doi: 10.3892/mmr.2018.9196. Epub 2018 Jun 20.
The aim of the present study was to investigate the eradicating effects of 460 nm blue light (BL) on Candida albicans in vitro and in C. albicans‑infected skin wounds in a mouse model. In the present study, the antifungal effects of irradiation with BL on C. albicans in vitro and in vivo were investigated. C. albicans colonies and cell numbers were investigated using the spread plate method and flow cytometry respectively following treatment with BL irradiation. In order to determine whether BL can eradicate C. albicans cells within biofilms, an in vitro C. albicans biofilm model was established, and the effect of BL was subsequently investigated using a confocal laser scanning microscope and a Live/Dead staining kit. Furthermore, a mouse skin wound infection model infected with C. albicans was established. Wound healing rates and histological examinations were determined 0, 3, 7, 10 and 14 days post‑wounding. The results revealed that C. albicans was eradicated by BL in a dose‑dependent manner, with a minimum fluence of 60 J/cm2. Irradiation with BL almost completely eradicated C. albicans when the light fluence was 240 J/cm2. C. albicans inside biofilms was also eradicated and biofilms were destroyed following BL irradiation at 240 J/cm2. In addition, BL was revealed to significantly suppress C. albicans infection in vivo. Irradiation with BL promoted the wound healing of C. albicans infected‑skin wounds in a mouse model. In conclusion, the results of the present study demonstrated that 460 nm BL may eradicate planktonic and biofilm C. albicans in vitro, and represents a novel therapeutic strategy for the treatment of C. albicans infections in vivo.
本研究旨在探讨 460nm 蓝光（BL）对体外和体内白色念珠菌的清除作用。本研究通过体外和体内实验研究了 BL 对白色念珠菌的抗真菌作用。用 BL 照射后，采用平板法和流式细胞术分别检测白色念珠菌的菌落和细胞数。为了确定 BL 是否能消除生物膜内的白色念珠菌细胞，建立了体外白色念珠菌生物膜模型，并用共聚焦激光扫描显微镜和 Live/Dead 染色试剂盒研究了 BL 的作用。此外，还建立了感染白色念珠菌的小鼠皮肤伤口感染模型。分别在伤后 0、3、7、10 和 14 天测定伤口愈合率和组织学检查。结果显示，白色念珠菌被 BL 以剂量依赖性方式清除，最低剂量为 60J/cm2。当光剂量为 240J/cm2 时，BL 几乎完全清除了白色念珠菌。当用 240J/cm2 的 BL 照射时，生物膜内的白色念珠菌也被清除，生物膜被破坏。此外，BL 还显著抑制了体内的白色念珠菌感染。BL 照射促进了白色念珠菌感染皮肤伤口在小鼠模型中的愈合。综上所述，本研究结果表明，460nm BL 可能清除浮游和生物膜形式的白色念珠菌，为治疗体内白色念珠菌感染提供了一种新的治疗策略。
