Opt Lett. 2018 Jul 1;43(13):3152-3155. doi: 10.1364/OL.43.003152.
While laser scanning fluorescence lifetime imaging (FLIM) is a powerful approach for cell biology, its small field of view (typically less than 1 mm) makes it impractical for the imaging of large biological samples that is often required for biomedical applications. Here we present a system that allows performing FLIM on macroscopic samples as large as 18 mm with a lateral resolution of 15 μm. The performance of the system is verified with FLIM of endogenous metabolic cofactor reduced nicotinamide adenine dinucleotide (phosphate), NAD(P)H, and genetically encoded fluorescent protein mKate2 in a mouse tumor in vivo.
虽然激光扫描荧光寿命成像（FLIM）是一种强大的细胞生物学方法，但由于其视场较小（通常小于 1 毫米），对于生物医学应用中经常需要的大型生物样品成像来说并不实用。在这里，我们展示了一种能够对 18 毫米大小的宏观样品进行 FLIM 成像的系统，其横向分辨率为 15 微米。该系统的性能通过对体内小鼠肿瘤中的内源性代谢辅因子还原型烟酰胺腺嘌呤二核苷酸（磷酸）（NAD(P)H）和遗传编码荧光蛋白 mKate2 的 FLIM 进行验证。
