慈济化癌保生方对H(22)肝癌小鼠模型中血管内皮生长因子、激酶插入结构域受体和碱性成纤维细胞生长因子表达的影响

Efficacy of Ciji Hua'ai Baosheng formula on the expressions of vascular endothelial growth factor, kinase insert domain-containing receptor and basic fibroblast growth factor in mouse models of H(22) hepatocellular carcinoma.

作者信息

Zhao Xinyue, Xi Shengyan, Wang Yanhui, Xu Yangxinzi, Pollock Galia, Su Yu, Cheng Yao, Loy Guanjie, Liu Pei

出版信息

J Tradit Chin Med. 2017 Feb;37(1):88-95. doi: 10.1016/s0254-6272(17)30031-6.

DOI:10.1016/s0254-6272(17)30031-6

PMID:29957915

Abstract

OBJECTIVE

To investigate the efficacy of Ciji Hua'ai Baosheng formula (CHBF) on microvessel density (MVD) and vascular endothelial growth factor (VEGF), kinase insert domain-containing receptor (KDR) and basic fibroblast growth factor (bFGF) expression in serum and tumor tissue of mice receiving chemotherapy for the treatment of H(22) hepatocellular carcinoma.

METHODS

Sixty Kunming mice were injected subcutaneously with H(22) hepatoma carcinoma cell suspensions into the right anterior armpit. Seven days later, all transplanted tumor were formed and the mice were intraperitoneally injected 200 mg/kg Cytoxan (CTX) to establish the models of tumor-bearing mouse chemotherapy, then they were randomly divided into model group, continuing CTX chemotherapy group (CTX group), and three CHBF (117, 58.5 and 29.25 g/kg) groups. After ten days of treatments, histology was observed, contents of VEGF, KDR and bFGF in serum and tumor tissue were measured by enzyme-linked immunosorbent assay (ELISA), VEGF and bFGF protein expression and MVD tagged by CD34 were detected by immunohistochemistry.

RESULTS

MVD in CHBF (117, 58.5 g/kg) and CTX groups was significantly lower than that in model group (P < 0.01); expressions of VEGF, KDR and bFGF in serum and tumor tissue in CHBF (117 g/kg) group were less than those in model group (P < 0.05; P < 0.01); the expressions of MVD, VEGF and bFGF in tumor tissue of CHBF (117 g/kg) group were also less than those in CTX group (P < 0.05; P < 0.01).

CONCLUSION

CHBF can effectively reduce the expression of VEGF, KDR and bFGF in serum and tumor tissue, and decrease MVD and delay tumor progression.

摘要

目的

探讨慈济化癌保生方（CHBF）对接受化疗的H(22)肝癌小鼠血清及肿瘤组织中微血管密度（MVD）、血管内皮生长因子（VEGF）、含激酶插入结构域受体（KDR）和碱性成纤维细胞生长因子（bFGF）表达的影响。

方法

60只昆明小鼠右前腋下皮下注射H(22)肝癌细胞悬液。7天后，所有移植瘤形成，小鼠腹腔注射200 mg/kg环磷酰胺（CTX）建立荷瘤小鼠化疗模型，然后随机分为模型组、继续CTX化疗组（CTX组）和3个CHBF（117、58.5和29.25 g/kg）组。治疗10天后，观察组织学变化，采用酶联免疫吸附测定（ELISA）法检测血清及肿瘤组织中VEGF、KDR和bFGF的含量，采用免疫组织化学法检测VEGF和bFGF蛋白表达及CD34标记的MVD。

结果

CHBF（117、58.5 g/kg）组和CTX组的MVD均显著低于模型组（P < 0.01）；CHBF（117 g/kg）组血清及肿瘤组织中VEGF、KDR和bFGF的表达均低于模型组（P < 0.05；P < 0.01）；CHBF（117 g/kg）组肿瘤组织中MVD、VEGF和bFGF的表达也低于CTX组（P < 0.05；P < 0.01）。