饮用果汁对人体代谢风险因素和基因表达谱的急性影响。

Acute Effects of the Consumption of Juice on Metabolic Risk Factors and Gene Expression Profile in Humans.

机构信息

Postgraduate Program in Human Nutrition, College of Health Sciences, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília DF 70.910-900, Brazil.

Unité de Nutrition Humaine, Université Clermont Auvergne, INRAE, UNH, F-63000 Clermont-Ferrand, France.

出版信息

Nutrients. 2020 Apr 16;12(4):1104. doi: 10.3390/nu12041104.

BACKGROUND

(PS) is a passionfruit variety of the Brazilian savannah and is a rich source of plant food bioactives with potential anti-inflammatory activity. This study aimed to investigate the effect of an acute intake of PS juice upon inflammation, metabolic parameters, and gene expression on circulating immune cells in humans.

METHODS

Overweight male volunteers ( = 12) were enrolled in two double-blind placebo-controlled studies. Blood samples were collected from fasting volunteers 3 h after the consumption of 250 mL of PS juice or placebo (PB). Metabolic parameters (insulin, glucose, total cholesterol, high-density lipoprotein (LDL), high-density lipoprotein (HDL), and total triglycerides) and circulating cytokines were evaluated (study 1). Peripheral blood mononuclear cell (PBMC) from the same subjects were isolated and RNA was extracted for transcriptomic analyses using microarrays (study 2).

RESULTS

Insulin and homeostatic model assessment for insulin resistance (HOMA-IR) levels decreased statistically after the PS juice intake, whereas HDL level increased significantly. Interleukin (IL)-17A level increased after placebo consumption, whereas its level remained unchanged after PS juice consumption. Nutrigenomic analyses revealed 1327 differentially expressed genes after PS consumption, with modulated genes involved in processes such as inflammation, cell adhesion, or cytokine-cytokine receptor.

CONCLUSION

Taken together, these clinical results support the hypothesis that PS consumption may help the prevention of cardiometabolic diseases.

背景

（PS）是巴西大草原的西番莲品种，是植物食品生物活性物质的丰富来源，具有潜在的抗炎活性。本研究旨在研究急性摄入 PS 汁对人体炎症、代谢参数和循环免疫细胞基因表达的影响。

方法

超重男性志愿者（n = 12）被纳入两项双盲安慰剂对照研究。在摄入 250 毫升 PS 汁或安慰剂（PB）后 3 小时，从禁食志愿者中采集血液样本。评估代谢参数（胰岛素、葡萄糖、总胆固醇、高密度脂蛋白（LDL）、高密度脂蛋白（HDL）和总甘油三酯）和循环细胞因子（研究 1）。从同一受试者中分离外周血单核细胞（PBMC），并使用微阵列提取 RNA 进行转录组分析（研究 2）。