University of Health Science, Faculty of Medicine, Department of Emergency Medicine, Trabzon, Turkey.
Karadeniz Technical University, Faculty of Medicine, Department of Medical Biochemistry, Trabzon, Turkey.
Am J Emerg Med. 2019 Apr;37(4):596-602. doi: 10.1016/j.ajem.2018.06.033. Epub 2018 Jun 19.
To evaluate levels of the endoplasmic reticulum (ER) stress markers GRP78 and CHOP in acute mesenteric ischemia (AMI) and to examine relations with degrees of AMI-related intestinal injury.
Twenty-four rats were divided into four groups. Group I and Group III represented the control groups, from which blood and tissue specimens were collected 2 and 6 h after laparotomy without superior mesenteric artery (SMA) ligation. Group II and Group IV constituted the ischemia groups, from which blood and tissue specimens were collected 2 and 6 h after SMA ligation. The ER stress markers GRP78 and CHOP, total oxidant status (TOS), total antioxidant status (TAS), and the oxidative stress index (OSI) were investigated in each group. Ileum specimens were assessed in terms of ischemic injury, and appropriate comparisons were performed.
Significantly higher GRP78, CHOP, TOS, and TAS values were determined in the ischemia groups (groups II and IV) compared to the control groups (groups I and III). This elevation was greater in the 6 h ischemia group, the group exposed to the greatest ischemic injury (Group IV). Significant and powerful correlation was present between histopathological damage and levels of the ER stress markers and oxidative markers.
According to our results, ER stress markers (GRP78 and CHOP) increase significantly following ischemic injury. This elevation has the potential to be used diagnostically and also in prognostic terms due to the powerful correlation it exhibits with AMI-related ischemic injury.
评估内质网（ER）应激标志物 GRP78 和 CHOP 在急性肠系膜缺血（AMI）中的水平，并研究其与 AMI 相关肠损伤程度的关系。
24 只大鼠分为 4 组。第 I 组和第 III 组为对照组，分别于剖腹后 2 小时和 6 小时采集血液和组织标本，不结扎肠系膜上动脉（SMA）。第 II 组和第 IV 组为缺血组，结扎 SMA 后 2 小时和 6 小时采集血液和组织标本。检测每组 ER 应激标志物 GRP78 和 CHOP、总氧化状态（TOS）、总抗氧化状态（TAS）和氧化应激指数（OSI）。根据缺血损伤评估回肠标本，并进行适当比较。
与对照组（第 I 组和第 III 组）相比，缺血组（第 II 组和第 IV 组）的 GRP78、CHOP、TOS 和 TAS 值显著升高。在 6 小时缺血组（暴露于最大缺血损伤的第 IV 组）中，这种升高更为明显。ER 应激标志物和氧化标志物的水平与组织病理学损伤之间存在显著且强大的相关性。
根据我们的结果，缺血损伤后 ER 应激标志物（GRP78 和 CHOP）显著增加。由于与 AMI 相关的缺血损伤表现出强大的相关性，这种升高具有诊断和预后的潜力。
