Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu, China.
Appl Environ Microbiol. 2018 Aug 17;84(17). doi: 10.1128/AEM.00920-18. Print 2018 Sep 1.
Abscisic acid (ABA) is one of the five classical phytohormones involved in increasing the tolerance of plants for various kinds of stresses caused by abiotic or biotic factors, and it also plays important roles in regulating the activation of innate immune cells and glucose homeostasis in mammals. For these reasons, as a "stress hormone," ABA has recently received attention as a candidate drug for agriculture and biomedical applications, prompting significant development of ABA synthesis. Some plant-pathogenic fungi can synthesize natural ABA. The fungus has been used for biotechnological production of ABA. Identification of the transcription factors (TFs) involved in regulation of ABA biosynthesis in would provide new clues to understand how ABA is synthesized and regulated. In this study, we defined a novel CysHis TF, BcabaR1, that regulates the transcriptional levels of ABA synthase genes (, , , and ) in an ABA-overproducing mutant, TBC-A. Electrophoretic mobility shift assays revealed that recombinant BcabaR1 can bind specifically to both a 14-nucleotide sequence motif and a 39-nucleotide sequence motif in the promoter region of to - genes A decreased transcriptional level of the gene in led to significantly decreased ABA production and downregulated transcription of to - When was overexpressed in , ABA production was significantly increased, with upregulated transcription of to - Thus, in this study, we found that BcabaR1 acts as a positive regulator of ABA biosynthesis in Abscisic acid (ABA) could make a potentially important contribution to theoretical research and applications in agriculture and medicine. is a plant-pathogenic fungus that was found to produce ABA. There has been a view that ABA is related to the interaction between pathogenic fungi and plants. Identification of regulatory genes involved in ABA biosynthesis may facilitate an understanding of the underlying molecular mechanisms of ABA biosynthesis and the pathogenesis of Here, we present a positive regulator, BcabaR1, of ABA biosynthesis in that can affect the transcriptional level of the ABA biosynthesis gene cluster, to -, by directly binding to the conserved sequence elements in the promoter of the to - genes. This TF was found to be specifically involved in regulation of ABA biosynthesis. This work provides new clues for finding other ABA biosynthesis genes and improving ABA yield in .
脱落酸（ABA）是参与提高植物对各种非生物或生物因素引起的胁迫的耐受性的五种经典植物激素之一，它在调节哺乳动物先天免疫细胞的激活和葡萄糖稳态方面也发挥着重要作用。由于这些原因，作为一种“应激激素”，ABA 最近作为农业和生物医学应用的候选药物受到关注，促使 ABA 的合成得到了显著发展。一些植物病原真菌可以合成天然 ABA。该真菌已被用于 ABA 的生物技术生产。鉴定参与 中 ABA 生物合成调节的转录因子（TFs）将为了解 ABA 的合成和调节提供新的线索。在这项研究中，我们定义了一个新的 CysHis TF，BcabaR1，它调节 ABA 合成酶基因（，，，和）在 ABA 过度产生突变体中的转录水平，TBC-A。电泳迁移率变动分析显示，重组 BcabaR1 可以特异性结合到 基因启动子区域的 14 个核苷酸序列基序和 39 个核苷酸序列基序中。在 中，基因的转录水平降低导致 ABA 产量显著降低，并下调了 到 -基因的转录。当 在 中过表达时，ABA 产量显著增加， 到 -基因的转录上调。因此，在这项研究中，我们发现 BcabaR1 作为 中 ABA 生物合成的正调节剂发挥作用。脱落酸（ABA）可能对农业和医学的理论研究和应用做出重要贡献。是一种植物病原真菌，被发现可以产生 ABA。有人认为 ABA 与致病真菌和植物之间的相互作用有关。鉴定参与 ABA 生物合成的调节基因可能有助于理解 ABA 生物合成的潜在分子机制和 的发病机制。在这里，我们提出了一个正调节剂，BcabaR1，在 中 ABA 生物合成，它可以通过直接结合到 到 -基因启动子中的保守序列元件来影响 ABA 生物合成基因簇的转录水平， 到 -。该 TF 被发现特异性参与 ABA 生物合成的调节。这项工作为寻找其他 ABA 生物合成基因和提高 在 中的 ABA 产量提供了新的线索。
