Zhang Ning, Yang Zhenzhou, Liang Wenxing, Liu Mengjie
Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
Front Microbiol. 2020 Oct 23;11:564350. doi: 10.3389/fmicb.2020.564350. eCollection 2020.
Lysine crotonylation (Kcr), a recently discovered post-translational modification, plays a key role in the regulation of diverse cellular processes. is a destructive necrotrophic fungal pathogen distributed worldwide with broad ranging hosts. However, the functions of Kcr are unknown in or any other plant fungal pathogens. Here, we comprehensively evaluated the crotonylation proteome of and identified 3967 Kcr sites in 1041 proteins, which contained 9 types of modification motifs. Our results show that although the crotonylation was largely conserved, different organisms contained distinct crotonylated proteins with unique functions. Bioinformatics analysis demonstrated that the majority of crotonylated proteins were distributed in cytoplasm (35%), mitochondria (26%), and nucleus (22%). The identified proteins were found to be involved in various metabolic and cellular processes, such as cytoplasmic translation and structural constituent of ribosome. Particularly, 26 crotonylated proteins participated in the pathogenicity of , suggesting a significant role for Kcr in this process. Protein interaction network analysis demonstrated that many protein interactions are regulated by crotonylation. Furthermore, our results show that different nutritional conditions had a significant influence on the Kcr levels of . These data represent the first report of the crotonylome of and provide a good foundation for further explorations of the role of Kcr in plant fungal pathogens.
赖氨酸巴豆酰化（Kcr）是一种最近发现的翻译后修饰，在多种细胞过程的调控中起关键作用。[病原菌名称]是一种具有破坏性的坏死营养型真菌病原体，分布于世界各地，寄主范围广泛。然而，Kcr在[病原菌名称]或任何其他植物真菌病原体中的功能尚不清楚。在此，我们全面评估了[病原菌名称]的巴豆酰化蛋白质组，并在1041个蛋白质中鉴定出3967个Kcr位点，这些位点包含9种修饰基序。我们的结果表明，尽管巴豆酰化在很大程度上是保守的，但不同生物体含有具有独特功能的不同巴豆酰化蛋白质。生物信息学分析表明，大多数巴豆酰化蛋白质分布在细胞质（35%）、线粒体（26%）和细胞核（22%）中。已鉴定出的蛋白质参与各种代谢和细胞过程，如细胞质翻译和核糖体的结构组成。特别地，26个巴豆酰化蛋白质参与了[病原菌名称]的致病性，表明Kcr在此过程中发挥了重要作用。蛋白质相互作用网络分析表明，许多蛋白质相互作用受巴豆酰化调控。此外，我们的结果表明，不同的营养条件对[病原菌名称]的Kcr水平有显著影响。这些数据代表了关于[病原菌名称]巴豆酰化蛋白质组的首次报道，并为进一步探索Kcr在植物真菌病原体中的作用提供了良好的基础。
