Niznik R A, Wideman R F, Cowen B S, Kissell R E
Poult Sci. 1985 Aug;64(8):1430-7. doi: 10.3382/ps.0641430.
Urolithiasis was induced in an experimental group of Single Comb White Leghorn pullets by feeding them layer ration and exposing them to nephrotrophic Gray strain infectious bronchitis virus (IBV). Gray strain IBV was recovered from kidney and cloacal swabs for up to 26 days after exposure to the virus. Control pullets fed pullet grower ration and not exposed to Gray strain IBV did not develop urolithiasis. The experimental design did not allow differentiation between the roles of layer ration and IBV in triggering urolithiasis. Urolithiasis was associated with asymmetry in left vs. right kidney weight comparisons for individual pullets. Pullets from the urolithiasis group had 43,800 +/- 4,500 glomeruli/gram kidney weight, whereas control pullets had 68,770 +/- 3,500 glomeruli/gram kidney weight. This difference was significant (P less than .01). Total kidney weights did not differ significantly when the experimental and control pullets were compared. Comparisons of glomeruli size distributions indicated that the number of intermediate sized glomeruli (.15 to .22 mm in circumference) was significantly reduced in pullets from the urolithiasis treatment group. These observations indicate that a significant reduction in nephron number can be masked by compensatory hypertrophy of the remaining kidney tissue in pullets with urolithiasis.
通过给单冠白来航母鸡实验组喂食蛋鸡日粮并使其接触嗜肾性灰色株传染性支气管炎病毒（IBV），诱导其发生尿石症。在接触该病毒后长达26天的时间里，从肾脏和泄殖腔拭子中都能分离出灰色株IBV。喂食雏鸡生长日粮且未接触灰色株IBV的对照母鸡未发生尿石症。该实验设计无法区分蛋鸡日粮和IBV在引发尿石症中的作用。尿石症与单个母鸡左右肾重量比较的不对称有关。尿石症组的母鸡每克肾脏重量有43,800±4,500个肾小球，而对照母鸡每克肾脏重量有68,770±3,500个肾小球。这种差异具有显著性（P小于0.01）。比较实验组和对照母鸡时，总肾脏重量没有显著差异。肾小球大小分布的比较表明，尿石症治疗组母鸡中中等大小肾小球（周长0.15至0.22毫米）的数量显著减少。这些观察结果表明，在患有尿石症的母鸡中，剩余肾脏组织的代偿性肥大可能掩盖了肾单位数量的显著减少。
