牛乳腺类器官：一种研究乳腺上皮细胞的模型。

Bovine Mammary Organoids: A Model to Study Epithelial Mammary Cells.

作者信息

Martignani Eugenio, Accornero Paolo, Miretti Silvia, Baratta Mario

机构信息

Department of Veterinary Science, University of Turin, Grugliasco, TO, Italy.

出版信息

Methods Mol Biol. 2018;1817:137-144. doi: 10.1007/978-1-4939-8600-2_14.

DOI:10.1007/978-1-4939-8600-2_14

PMID:29959710

Abstract

Bovine mammary organoids are cell aggregates that are produced by an association of a mechanical and an enzymatic dissociation of mammary gland tissue. They provide a useful source to isolate mammary epithelial cells, but can also be frozen as an intermediate dissociation step.Due to the strong cell-cell interactions among epithelial cells, the production and isolation of organoids is an efficient way to remove unwanted cell population of non-epithelial origin like fibroblasts.

摘要

牛乳腺类器官是通过乳腺组织的机械解离和酶解离相结合产生的细胞聚集体。它们为分离乳腺上皮细胞提供了有用的来源，但也可以作为中间解离步骤进行冷冻保存。由于上皮细胞之间存在强烈的细胞间相互作用，类器官的产生和分离是去除成纤维细胞等非上皮来源的不需要细胞群体的有效方法。