通过对三联比对的蛋白质序列进行评分来定位痘苗病毒和单纯疱疹病毒胸苷激酶中的核苷酸结合位点。
Locating a nucleotide-binding site in the thymidine kinase of vaccinia virus and of herpes simplex virus by scoring triply aligned protein sequences.
作者信息
Gentry G A
出版信息
Proc Natl Acad Sci U S A. 1985 Oct;82(20):6815-9. doi: 10.1073/pnas.82.20.6815.
Abstract
Computer techniques were used to locate related segments of amino acid sequences in the thymidine kinases of vaccinia virus and of herpes simplex virus type 1 and in porcine adenylate kinase. As determined by a procedure that evaluates triply aligned sequences, the probability that the similarities among the segments described here arose by chance was no greater than 0.001. Because the sequence in porcine adenylate kinase is a nucleotide phosphate-binding site it is concluded that the segments in the vaccinia virus and herpes simplex virus thymidine kinases perform similar functions. The segments are residues 16-23 in porcine adenylate kinase, 11-19 in vaccinia virus thymidine kinase, and 56-64 in herpes simplex virus thymidine kinase.
摘要
利用计算机技术在痘苗病毒和1型单纯疱疹病毒的胸苷激酶以及猪腺苷酸激酶中定位氨基酸序列的相关片段。通过评估三重比对序列的程序确定，此处描述的片段之间的相似性偶然出现的概率不超过0.001。由于猪腺苷酸激酶中的序列是一个核苷酸磷酸结合位点，因此得出结论，痘苗病毒和单纯疱疹病毒胸苷激酶中的片段执行相似的功能。这些片段分别是猪腺苷酸激酶中的第16 - 23位残基、痘苗病毒胸苷激酶中的第11 - 19位残基以及单纯疱疹病毒胸苷激酶中的第56 - 64位残基。
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