State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
New Phytol. 2019 Jan;221(1):295-308. doi: 10.1111/nph.15306. Epub 2018 Jun 30.
The accumulation of anthocyanins in response to specific developmental cues or environmental conditions plays a vital role in plant development and protection against stresses. Extensive research has examined the regulation of anthocyanin biosynthetic genes at the transcriptional and post-transcriptional levels, but the role of chromatin in this regulation remains unknown. Chromatin immunoprecipitation and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses were performed. Genetic interactions between trimethylation of lysine 4 on histone H3 (H3K4me3) and the chromatin remodeling complex SWR1 in the control of anthocyanin biosynthesis were further studied. In this study, we provide evidence that a conserved histone H2 variant, H2A.Z, negatively regulates anthocyanin accumulation through deposition at a set of anthocyanin biosynthetic genes and consequently represses their expression in Arabidopsis thaliana. Our data indicate that the accumulation of anthocyanin in H2A.Z deposition-deficient mutants is associated with increased H3K4me3, which is required for promotion of the expression of anthocyanin biosynthetic genes. We further provide evidence that H3K4me3 in anthocyanin biosynthetic genes is negatively associated with the presence of H2A.Z. Our results reveal an antagonistic relationship between H2A.Z and H3K4me3 in the regulation of the expression of anthocyanin biosynthesis genes, adding another layer of regulation to anthocyanin biosynthesis genes and highlighting the role of chromatin in gene regulation.
花青素的积累是对特定发育线索或环境条件的响应，在植物发育和抵御胁迫中起着至关重要的作用。大量研究已经检查了在转录和转录后水平上花青素生物合成基因的调控，但染色质在这种调控中的作用仍然未知。进行了染色质免疫沉淀和定量逆转录聚合酶链反应（qRT-PCR）分析。进一步研究了组蛋白 H3 赖氨酸 4 三甲基化（H3K4me3）和染色质重塑复合物 SWR1 之间的遗传相互作用在花青素生物合成控制中的作用。在这项研究中，我们提供了证据表明，一种保守的组蛋白 H2 变体 H2A.Z 通过沉积在一组花青素生物合成基因上来负调控花青素的积累，从而抑制拟南芥中这些基因的表达。我们的数据表明，在 H2A.Z 沉积缺陷突变体中花青素的积累与 H3K4me3 的增加有关，H3K4me3 是促进花青素生物合成基因表达所必需的。我们进一步提供了证据表明，花青素生物合成基因中的 H3K4me3 与 H2A.Z 的存在呈负相关。我们的结果揭示了 H2A.Z 和 H3K4me3 在花青素生物合成基因表达调控中的拮抗关系，为花青素生物合成基因的调控增加了另一层调控，并强调了染色质在基因调控中的作用。
