Egrie J C, Browne J, Lai P, Lin F K
Prog Clin Biol Res. 1985;191:339-50.
We have isolated the human EPO gene from a human genomic lambda library and a monkey EPO cDNA from an anemic monkey kidney cDNA library. These sequences have been expressed in both COS1 and Chinese hamster ovary cells. The expressed EPO has been shown to be fully biologically active both in vitro and in vivo and immunologically identical to the native hormone. In addition, administration of recombinant EPO to animals causes a dramatic increase in their hematocrit.
我们已从人基因组λ文库中分离出人类促红细胞生成素（EPO）基因，并从贫血猴肾cDNA文库中分离出猴EPO cDNA。这些序列已在COS1细胞和中国仓鼠卵巢细胞中表达。所表达的EPO在体外和体内均显示出完全的生物活性，并且在免疫学上与天然激素相同。此外，给动物施用重组EPO会使其血细胞比容显著增加。
