Wang Yili, Tian Yuanxiang, Zhao Jianxin, Xu Lei
J Tradit Chin Med. 2017 Apr;37(2):252-60. doi: 10.1016/s0254-6272(17)30052-3.
To observe the regulation of electroacupuncture on gene expression at calcium signaling pathways in mice with cerebral ischemia reperfusion.
Sixty male, inbred Kunming mice were randomly assigned to three groups: repeated cerebral ischemia reperfusion group (RG, n = 24), sham-operated group (SG, n = 12), and electroacupuncture group (EG, n = 24). Mice in RG and EG groups were modeled by repeated cerebral ischemia reperfusion surgery, and EG mice were treated with electroacupuncture for 30 min after recovery from anesthesia. Changes in gene expression profile of mice hippocampi were analyzed by global expression profile microarray. Genes that were up-regulated or down-regulated greater than 1.5 folds were considered to be biologically meaningful. Real-time quantitative polymerase chain reaction (q-PCR) method was used to verify the expression of selected genes based on the algorithm [2^ (ΔΔCt)].
Compared with SG mice, 242 genes showed different in expressions in RG mice: 107 down-regulated and 135 up-regulated. Compared with RG mice, 609 genes showed a difference of expression in EG mice: 315 down-regulated and 375 up-regulated. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated two pathways: calcium signaling and long-term potentiation in which 11 differentially expressed genes selected. Six of the 11 genes in the calcium signaling pathway were verified after real-time q-PCR testing.
Electroacupuncture treatment of cerebral ischemia reperfusion appears to regulate Atp2a2, Cacna1e, Camk2a, Gnas, Grm1, Rapgef3 genes in the calcium signaling pathway.
观察电针对脑缺血再灌注小鼠钙信号通路基因表达的调控作用。
将60只雄性近交系昆明小鼠随机分为三组：反复脑缺血再灌注组（RG，n = 24）、假手术组（SG，n = 12）和电针组（EG，n = 24）。RG组和EG组小鼠采用反复脑缺血再灌注手术造模，EG组小鼠在麻醉苏醒后接受30分钟电针治疗。通过全基因组表达谱芯片分析小鼠海马基因表达谱的变化。上调或下调超过1.5倍的基因被认为具有生物学意义。采用实时定量聚合酶链反应（q-PCR）方法，根据[2^（ΔΔCt）]算法验证所选基因的表达。
与SG组小鼠相比，RG组小鼠有242个基因表达差异：107个下调，135个上调。与RG组小鼠相比，EG组小鼠有609个基因表达差异：315个下调，375个上调。基因本体论和京都基因与基因组百科全书分析表明有两条通路：钙信号通路和长时程增强，从中选择了11个差异表达基因。实时q-PCR检测验证了钙信号通路中11个基因中的6个。
电针治疗脑缺血再灌注似乎可调节钙信号通路中的Atp2a2、Cacna1e、Camk2a、Gnas、Grm1、Rapgef3基因。
