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针刺通过调节大电导钙激活钾通道的开放改善脑缺血再灌注大鼠的神经功能。

Acupuncture ameliorates neurological function in rats with cerebral ischemia-reperfusion by regulating the opening of large-conductance Ca -activated potassium channels.

机构信息

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.

National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, China.

出版信息

Brain Behav. 2021 Aug;11(8):e2286. doi: 10.1002/brb3.2286. Epub 2021 Aug 1.

DOI:10.1002/brb3.2286
PMID:34333869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8413763/
Abstract

Acupuncture has a good effect on improving neurological function after cerebral ischemia-reperfusion, but there are few studies on the neuroprotective effect of acupuncture from the perspective of ion channel cellular electrophysiology. Studies have shown that the over activation of large-conductance Ca -activated potassium channel （BKCa） after cerebral ischemia-reperfusion can reduce the excitability of neurons and induce apoptosis. This study intends to establish middle cerebral artery occlusion/reperfusion (MCAO/R) model, with acupuncture at GV26 as the intervention measure, using patch-clamp technique to record the electrophysiological changes of BKCa channel. The results showed that the neurological function score of MCAO/R rats was significantly decreased, and the conductance, open dwell time and open probability of BKCa channel in hippocampal CA1 neurons of MCAO/R rats were significantly increased. Acupuncture at GV26 could significantly improve the neurological function scores of MCAO/R rats, and reduce the conductance, open dwell time, and open probability of BKCa channel. The effect of acupuncture at GV26 was significantly better than acupuncture at non-acupuncture point. The neuroprotective effect of acupuncture at GV26 after cerebral ischemia-reperfusion may be related to regulating the electrophysiological characteristics of BKCa channel opening.

摘要

针刺对脑缺血再灌注后改善神经功能有较好的效果，但从离子通道细胞电生理学角度探讨针刺对脑缺血神经保护作用的研究较少。研究表明，脑缺血再灌注后大电导钙激活钾通道（BKCa）的过度激活可降低神经元的兴奋性并诱导细胞凋亡。本研究拟采用大脑中动脉闭塞/再灌注（MCAO/R）模型，以针刺百会穴（GV26）为干预措施，应用膜片钳技术记录 BKCa 通道的电生理变化。结果显示，MCAO/R 大鼠的神经功能评分显著降低，MCAO/R 大鼠海马 CA1 神经元 BKCa 通道的电导、开放时间和开放概率显著增加。针刺 GV26 可显著改善 MCAO/R 大鼠的神经功能评分，降低 BKCa 通道的电导、开放时间和开放概率。针刺 GV26 的效果明显优于针刺非穴位。针刺 GV26 对脑缺血再灌注后神经保护作用可能与调节 BKCa 通道开放的电生理特性有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/8784007b96ac/BRB3-11-e2286-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/e72b3c5bec4a/BRB3-11-e2286-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/888d680ebaa0/BRB3-11-e2286-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/96791b8f6078/BRB3-11-e2286-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/b6478742c7f2/BRB3-11-e2286-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/41c4dd92aa01/BRB3-11-e2286-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/8784007b96ac/BRB3-11-e2286-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/e72b3c5bec4a/BRB3-11-e2286-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/888d680ebaa0/BRB3-11-e2286-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/96791b8f6078/BRB3-11-e2286-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/b6478742c7f2/BRB3-11-e2286-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/41c4dd92aa01/BRB3-11-e2286-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e3/8413763/8784007b96ac/BRB3-11-e2286-g003.jpg

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