Starich T, Cordes P, Zissler J
Science. 1985 Nov 1;230(4725):541-3. doi: 10.1126/science.2996138.
Transposon mutagenesis of the bacterium Myxococcus xanthus with the transposon Tn5 revealed a special class of bacterial mutants that transduced the transposon through culture supernatant fluids. Virus-like particles copurified with transducing activity. Transposon tagging for detecting these virus-like particles may be generally useful in isolating endogenous viral agents capable of transferring genetic information between cells.
用转座子Tn5对黄色粘球菌进行转座子诱变,发现了一类特殊的细菌突变体,它们通过培养上清液转导转座子。与转导活性共纯化的病毒样颗粒。用于检测这些病毒样颗粒的转座子标签可能普遍有助于分离能够在细胞间传递遗传信息的内源性病毒因子。