Saulnier P, Hanquier J, Jaoua S, Reichenbach H, Guespin-Michel J F
Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, France.
J Gen Microbiol. 1988 Nov;134(11):2889-95. doi: 10.1099/00221287-134-11-2889.
No free plasmid has ever been found in the myxobacterium Myxococcus xanthus, but IncP-1 plasmids are able to integrate into the chromosome of this bacterium. The frequency of integration depends greatly upon the structure of the IncP-1 plasmid used. This property has been used to devise new delivery systems for transposon mutagenesis in this species. Plasmids with low integration efficiencies have proved to be efficient donors of Tn5, while plasmids with very high frequencies of integration could be used directly to generate mutations. These vectors have also proved efficient for Tn5 transfer into other species of myxobacteria, which have not so far been susceptible to genetic analysis.
在粘细菌黄色粘球菌中从未发现过游离质粒，但IncP-1质粒能够整合到该细菌的染色体中。整合频率在很大程度上取决于所使用的IncP-1质粒的结构。这一特性已被用于设计该物种中转座子诱变的新递送系统。整合效率低的质粒已被证明是Tn5的有效供体，而整合频率非常高的质粒可直接用于产生突变。这些载体也已被证明在将Tn5转移到其他粘细菌物种中是有效的，而这些物种迄今为止对遗传分析不敏感。
