Chen H, Keseler I M, Shimkets L J
Department of Microbiology, University of Georgia, Athens 30602.
J Bacteriol. 1990 Aug;172(8):4206-13. doi: 10.1128/jb.172.8.4206-4213.1990.
Genomic DNA of the myxobacterium Myxococcus xanthus was digested with the rare cutting restriction endonuclease AseI or SpeI, and the restriction products were separated by pulsed-field gel electrophoresis. Transposons Tn5-132 and Tn5 lac, which contain AseI restriction sites, were used to determine the number of restriction fragments in each band. The size of the genome was determined by adding the molecular sizes of the restriction products. The genomes of strains DK101, MD2, and DZF1 have identical restriction patterns and were estimated to be 9,454 +/- 101 kilobase pairs from the AseI digestions and 9,453 +/- 106 kilobase pairs from the SpeI digestions. DK1622, which was derived from DK101 by treatment with UV light, has suffered a 220- to 222-kilobase-pair deletion that removed an AseI and an SpeI restriction site. The deleted DNA may consist exclusively of Mx alpha-associated sequences.
用稀有切割限制酶AseI或SpeI消化粘细菌黄色粘球菌的基因组DNA，并用脉冲场凝胶电泳分离限制产物。含有AseI限制位点的转座子Tn5 - 132和Tn5 lac用于确定每条带中限制片段的数量。通过将限制产物的分子大小相加来确定基因组的大小。菌株DK101、MD2和DZF1的基因组具有相同的限制图谱，根据AseI消化估计为9454±101千碱基对，根据SpeI消化估计为9453±106千碱基对。通过紫外线处理从DK101衍生而来的DK1622发生了220至222千碱基对的缺失，该缺失去除了一个AseI和一个SpeI限制位点。缺失的DNA可能仅由与Mxα相关的序列组成。
