Brennan Todd V, Lin Liwen, Huang Xiaopei, Yang Yiping
Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Department of Medicine, Duke University Medical Center, Durham, NC, USA.
Bio Protoc. 2018 Apr 20;8(8). doi: 10.21769/BioProtoc.2817.
Murine tumor models have been critical to advances in our knowledge of tumor physiology and for the development of effective tumor therapies. Essential to these studies is the ability to both track tumor development and quantify tumor burden . For this purpose, the introduction of genes that confer tumors with bioluminescent properties has been a critical advance for oncologic studies in rodents. Methods of introducing bioluminescent genes, such as firefly luciferase, by viral transduction has allowed for the production of tumor cell lines that can be followed longitudinally over long periods of time. Here we describe methods for the production of stable luciferase expressing tumor cell lines by lentiviral transduction.
小鼠肿瘤模型对于我们在肿瘤生理学知识方面的进展以及有效肿瘤治疗方法的开发至关重要。这些研究的关键在于能够追踪肿瘤发展并量化肿瘤负荷。为此，引入赋予肿瘤生物发光特性的基因是啮齿动物肿瘤学研究的一项关键进展。通过病毒转导引入生物发光基因（如萤火虫荧光素酶）的方法，使得能够产生可长期纵向追踪的肿瘤细胞系。在这里，我们描述了通过慢病毒转导产生稳定表达荧光素酶的肿瘤细胞系的方法。
