Ollodart Jenna, Contino Kelly F, Steele Laiton R, Yu Yang, Shiozawa Yusuke
Department of Cancer Biology, Wake Forest University School of Medicine, and Atrium Health Wake Forest Baptist Comprehensive Cancer, Winston-Salem, NC, USA.
Methods Mol Biol. 2025;2885:423-432. doi: 10.1007/978-1-0716-4306-8_22.
More than 80% of patients with advanced prostate cancer experience bone metastasis, which negatively impacts overall survival and patients' quality of life. To elucidate underlying mechanisms and devise effective treatments for bone-metastatic disease, the development of animal models is imperative. However, fully replicating human conditions proves challenging as bone metastasis rarely occurs in spontaneous prostate cancer mouse models. Therefore, to investigate the interaction between bone-metastatic cancer cells and the bone microenvironment, mouse models with direct intraosseous injection (e.g., intrafemoral injection) have been widely used. Assessing bone remodeling is key to understanding the underlying mechanisms and pathology behind this disease. As such, longitudinal radiographic analysis for assessing progressive structural changes of the bone has been used since this method presents as a more cost-effective, accessible imaging modality compared to magnetic resonance imaging and computed tomography. Here, we describe a method of analysis that primarily focuses on scoring the osteoblastic phenotype (solid and spiculated) in our prostate cancer bone-metastatic mouse models, given the predominance of osteosclerotic activity in prostate cancer patients.
超过80%的晚期前列腺癌患者会发生骨转移，这对总生存期和患者生活质量产生负面影响。为了阐明潜在机制并设计出针对骨转移性疾病的有效治疗方法，动物模型的开发势在必行。然而，由于自发性前列腺癌小鼠模型中很少发生骨转移，要完全复制人类情况具有挑战性。因此，为了研究骨转移性癌细胞与骨微环境之间的相互作用，直接骨内注射（如股内注射）的小鼠模型已被广泛使用。评估骨重塑是理解该疾病潜在机制和病理的关键。因此，由于与磁共振成像和计算机断层扫描相比，纵向放射学分析是一种更具成本效益、更易获得的成像方式，所以一直被用于评估骨的渐进性结构变化。在此，鉴于前列腺癌患者中骨硬化活性占主导，我们描述一种主要侧重于对我们前列腺癌骨转移小鼠模型中的成骨细胞表型（实性和针状）进行评分的分析方法。
