Mazumder R, Das T K, Biswas N M
Andrologia. 1985 Jul-Aug;17(4):400-5. doi: 10.1111/j.1439-0272.1985.tb01029.x.
Spermatogenesis and steroidogenesis of male Wistar rats under the influence of a decreased central NE level was studied. Inhibition of NE synthesis was produced by chronic injection (7 days) of DDC, a Dopamine hydroxylase blocker, which decreased brain NE, increased brain DA without significantly affecting brain 5-HT. Rats were sacrificed on the day after cessation of treatment (8th day) and after an interval of 13 days following the cessation of treatment (21st day). The time interval of 13 days is equivalent to one cycle of the seminiferous epithelium in Wistar strain rats. A degenerative change (reduced spermatid count) in the spermatogenic pattern at stage-VII of the cycle of the seminiferous epithelium was observed in the rats sacrificed on the 8th day, the degeneration being much greater in the rats sacrificed on the 21st day. Rats sacrificed on the day after cessation of DDC treatment revealed an inhibition of plasma testosterone level. Such change was not observed in the rats sacrificed after the interval of 13 days following DDC treatment. Human chorionic gonadotropin supplementation in the rats sacrificed on the 21st day partially restored the spermatogenesis towards the vehicle treated control. The inhibition of spermatogenesis and steroidogenesis reflects a decrease in the pituitary gonadotropin secretion under the influence of a decreased NE synthesis in brain, following chronic treatment of DDC.
研究了中枢去甲肾上腺素（NE）水平降低对雄性Wistar大鼠精子发生和类固醇生成的影响。通过多巴胺羟化酶阻滞剂DDC的慢性注射（7天）来抑制NE合成，这降低了脑内NE水平，增加了脑内多巴胺（DA）水平，而对脑内5-羟色胺（5-HT）没有显著影响。在停止治疗后的第2天（第8天）以及停止治疗后的13天间隔期（第21天）处死大鼠。13天的时间间隔相当于Wistar品系大鼠生精上皮的一个周期。在第8天处死的大鼠中，观察到生精上皮周期VII期的生精模式出现退行性变化（精子细胞数量减少），在第21天处死的大鼠中这种退化更为严重。在停止DDC治疗后的第2天处死的大鼠显示血浆睾酮水平受到抑制。在DDC治疗后的13天间隔期后处死的大鼠中未观察到这种变化。对第21天处死的大鼠补充人绒毛膜促性腺激素可使精子发生部分恢复至与溶剂处理对照组相似的水平。精子发生和类固醇生成的抑制反映了在长期使用DDC治疗后，脑内NE合成减少的影响下，垂体促性腺激素分泌减少。
