Das T K, Mazumder R, Biswas N M
Andrologia. 1982 May-Jun;14(3):242-9. doi: 10.1111/j.1439-0272.1982.tb02252.x.
The present experiment was designed to assess the role of enhanced central 5-HT level on testicular steroidogenesis and spermatogenesis of rat. L-tryptophan, a precursor in the synthesis of 5-HT in brain, was injected in male rats on a short-term (7-days) and a long-term (21-days) basis. The short-term treatment had no effect on the spermatogenic pattern (Stage - VII of the cycle of the seminiferous epithelium), while the long-term treatment showed a degenerative change (reduced count of the germ cells). Both modes of treatment reduced the activity of testicular delta 5-3 beta-HSD without affecting NADH2-linked Diaphorase activity. Histological study of the Leydig cells' nuclear area also revealed a marked reduction after both the modes of treatment. Human chorionic gonadotropin supplementation in the long-term treatment restored the spermatogenesis, delta 5-3 beta-HSD activity and Leydig cells' nuclear area towards the vehicle treated control. The inhibition of spermatogenesis and steroidogenesis reflects a decrease in the pituitary gonadotropin release under the influence of enhanced 5-HT synthesis in brain, after L-tryptophan loading.
本实验旨在评估中枢5-羟色胺（5-HT）水平升高对大鼠睾丸类固醇生成和精子发生的作用。L-色氨酸是大脑中5-HT合成的前体，对雄性大鼠进行短期（7天）和长期（21天）注射。短期治疗对精子发生模式（生精上皮周期的VII期）没有影响，而长期治疗则显示出退行性变化（生殖细胞数量减少）。两种治疗方式均降低了睾丸δ5-3β-羟类固醇脱氢酶（δ5-3β-HSD）的活性，但不影响与NADH2相关的黄递酶活性。对睾丸间质细胞核面积的组织学研究也显示，两种治疗方式后核面积均显著减小。长期治疗中补充人绒毛膜促性腺激素可使精子发生、δ5-3β-HSD活性和睾丸间质细胞核面积恢复到溶剂处理对照组的水平。在L-色氨酸负荷后，精子发生和类固醇生成的抑制反映了在大脑中5-HT合成增强的影响下垂体促性腺激素释放的减少。
