Cid-Uribe Jimena I, Santibáñez-López Carlos E, Meneses Erika P, Batista Cesar V F, Jiménez-Vargas Juana M, Ortiz Ernesto, Possani Lourival D
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Apartado Postal 510-3, Cuernavaca, Morelos, 62210, Mexico.
Laboratorio Universitario de Proteómica, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Apartado Postal 510-3, Cuernavaca, Morelos, 62210, Mexico.
Toxicon. 2018 Sep 1;151:47-62. doi: 10.1016/j.toxicon.2018.06.085. Epub 2018 Jun 28.
The recent publication of high-throughput transcriptomic and proteomic analyses of scorpion venom glands has increased our knowledge on the biodiversity of venom components. In this contribution, we report the transcriptome of the venom gland and the proteome of the venom for the scorpion species Paravaejovis schwenkmeyeri, a member of the family Vaejovidae. We report 138 annotated transcripts encoding putative peptides/proteins with sequence identity to known venom components available from different databases. A fingerprint analysis containing the molecular masses of 212 components of the whole soluble venom revealed molecular weights of approximately 700 to 13,800 Da, with most detected proteins ranging from 1500 to 3000 Da. Amino acid sequencing of venom components by LC-MS/MS allowed the identification of fragments from 27 peptides encoded by transcripts found in the transcriptome analysis. Enzymatic assays conducted with the soluble venom fraction confirmed the presence of enzymes such as hyaluronidases and phospholipases. The database presented here increases our general knowledge on the biodiversity of venom components from neglected non-buthid scorpions.
最近关于蝎毒腺的高通量转录组学和蛋白质组学分析的发表，增加了我们对毒液成分生物多样性的了解。在本论文中，我们报告了蝎种施氏副杀牛蝎（Paravaejovis schwenkmeyeri）的毒腺转录组和毒液蛋白质组，施氏副杀牛蝎属于杀牛蝎科（Vaejovidae）。我们报告了138个注释转录本，它们编码与不同数据库中已知毒液成分具有序列同一性的假定肽/蛋白质。对整个可溶性毒液212种成分的分子量进行的指纹分析显示，分子量约为700至13,800道尔顿，大多数检测到的蛋白质分子量在1500至3000道尔顿之间。通过液相色谱-串联质谱（LC-MS/MS）对毒液成分进行氨基酸测序，能够鉴定出转录组分析中发现的转录本所编码的27种肽的片段。对可溶性毒液部分进行的酶活性测定证实了透明质酸酶和磷脂酶等酶的存在。本文提供的数据库增加了我们对被忽视的非钳蝎科蝎子毒液成分生物多样性的总体认识。
