He Long, Li Yan-Qin, Li Bin-Chun, Li Jun-Jian
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
Institute of Loess Plateau, Shanxi University, Taiyuan 030006, China.
Huan Jing Ke Xue. 2017 Feb 8;38(2):752-759. doi: 10.13227/j.hjkx.201607200.
Effects of different vegetation types (, , , and ) and reclamation years (15 and 20 years) on soil bacterial community structure in reclaimed Antaibao opencast mine areas were investigated using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and clone sequencing. For 20-year reclaimed soils, the significantly highest and lowest bacterial diversity were found in and stand, respectively, whereas no significant differences were found between the other three vegetation types. Under 15-year plantations, soil bacterial diversity index of was significantly higher than that of . Soil bacterial diversity index significantly increased in planted soils but decreased in treatment with the increase of reclaimed years. No significant change of soil bacterial community structure was observed in the same reclamation years based on the similarity coefficient analysis, cluster analysis and principal component analysis (PCA). Pearson correlation analysis demonstrated that bacterial diversity index was significantly positively correlated with soil pH. , , , , as well as which play important roles in the nitrogen cycle, degradation of polycyclic aromatic hydrocarbons and other organic matter, were identified by clone sequencing of the DGGE bands. Our results indicated that and were conducive to the recovery of soil bacterial diversity. The most dominant bacterial community from reclaimed mine soil would be beneficial for restoring wasteland contaminated soil and improving soil fertility.
利用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）和克隆测序技术，研究了不同植被类型（，，，和）以及复垦年限（15年和20年）对安太堡露天煤矿复垦区土壤细菌群落结构的影响。对于复垦20年的土壤，细菌多样性显著最高和最低的分别出现在和林分中，而其他三种植被类型之间未发现显著差异。在15年的人工林条件下，的土壤细菌多样性指数显著高于。随着复垦年限的增加，种植土壤中的细菌多样性指数显著增加，而处理中的细菌多样性指数下降。基于相似系数分析、聚类分析和主成分分析（PCA），在相同的复垦年限内未观察到土壤细菌群落结构的显著变化。Pearson相关分析表明，细菌多样性指数与土壤pH值显著正相关。通过对DGGE条带的克隆测序，鉴定出了在氮循环、多环芳烃降解和其他有机物降解中起重要作用的，，，，以及。我们的结果表明，和有利于土壤细菌多样性的恢复。复垦矿山土壤中最主要的细菌群落将有利于恢复荒地污染土壤和提高土壤肥力。
